P30A: Biomaterials Core (6 of 10)

生物材料和组织工程在生殖生物学中的应用提供了一种使 促进肿瘤生育联盟发展的技术基础。正在开发一种核心，用于 这项技术的翻译。这项拟议的核心有两个主要任务：i)研究以进一步开发这一技术 使技术能够识别冷冻保存和成熟的灵长类卵泡的条件，ii)服务于 为成熟程序向卫星地点提供材料和培训。以前的工作有 证明了水凝胶海藻酸盐通过维持卵泡来模拟体内环境 架构，同时呈现可扩散、不可溶解和机械信号的组合，这些信号结合在一起 影响卵泡的发育。卵巢卵泡可以在体外成熟以产生高质量的卵母细胞 它们可以受精，并支持与小鼠的活产。本课题的研究目标是有针对性的。 由于需要开发水凝胶，i)支持大型灵长类毛囊的生长，这些毛囊可能具有 对小鼠卵泡的不同要求，ii)维持卵泡的结构并便于在 冷冻保存，以及iii)促进移植后移植卵泡的植入和存活。 因此，这一核心的活动侧重于四个具体目标。目标1：提供生物材料支持 肿瘤生育联盟(R01A、R01B、R01C、P30A)。目标2：确定生物材料的特性和培养 最大限度地促进灵长类卵泡生长的条件，这将转化为R01B和R01C。目标3： 开发可用于将组织冷冻损伤降至最低的新型生物材料，以转化为R01A和P30B。 目标4：设计药物释放水凝胶以优化皮质条移植(R01B)。一支令人兴奋的团队 研究人员已经聚集在一起，准备将新技术转化为重要的临床 有问题。重要的是，可受精卵母细胞和健康胚胎的产生可以立即 在现有的体外受精基础设施的基础上，翻译成临床应用。

The application of biomaterials and tissue engineering to reproductive biology provides an enabling technology underlying the development of the Oncofertility Consortium. A core is being developed for the translation of this technology.This proposed core has two major missions: i) research to further develop this enabling technology identify conditions for cryopreserving and maturing primate ovarian follicles, ii) service to provide materials and training to satellite locations for the maturation procedures. Previous work has demonstrated that the hydrogel alginate phenocopies the in vivo environment by maintaining follicular architecture while presenting a combination of diffusible, insoluble, and mechanical signals that combine to influence the development of the follicle. Ovarian follicles can be matured in vitro to yield high quality oocytes that can be fertilized and support live births with mice. The research objectives of this project are motivated by the need to develop hydrogels that i) support the growth of the large primate follicles that may have different requirements from mouse follicles, ii) maintain the follicle architecture and facilitate handling during cryopreservation, and iii) promote engraftment and survival of transplanted follicles following transplantation. The activities of this core are thus focused on four specific goals. Goal 1: Provide biomaterial support to the oncofertility consortium (R01A, R01B, R01C, P30A). Goal 2: Identify biomaterial properties and culture conditions that will maximize primate follicle growth, which will be translated to R01B and R01C. Goal 3: Develop novel biomaterials that can be used to minimize tissue cryoinjury for translation to R01A and P30B. Goal 4: Engineer drug-releasing hydrogels to optimize cortical strip transplants (R01B). An exciting team of investigators has been assembled towards translating novel technologies towards a significant clinical problem. Importantly, the generation of fertilizable oocytes and healthy embryos can immediately be translated clinically given the existing infrastructure for in vitro fertilization.