Skin Phenotyping Core

皮肤病研究中心的皮肤表型核心提供了广泛的组织学， 免疫组织化学和基于分子的工具来表征大体和微观形态， 突变体和基因工程实验动物皮肤的功能特征和基因表达。 在这个核心的特殊技术专长包括皮肤和毛囊形态的广泛知识 对于理解基因型/表型相关性至关重要;基因表达的最新方法 针对小鼠和人类皮肤专门设计的mRNA和蛋白质水平的表达研究;以及 拥有一系列原始的实验方法来研究体内皮肤生理学。核心 将协助中心的研究人员以及哥伦比亚的其他研究人员评估一个 基因突变、药物或其他实验性药物的表型表现或意外皮肤效应 操纵;了解皮肤/毛发中涉及的基因和分子途径的可能功能 表型的发展，并分析研究人员感兴趣的基因的皮肤表达模式， mRNA和蛋白水平采用免疫组织化学和原位杂交。此外，核心将 为研究者提供出版物材料与方法章节的校对阅读和建议 以及利用皮肤表型分析的校外研究基金的申请。皮肤表型 核心还作为调查人员之间的沟通中心，为交流 样品、抗体、技术和想法。核心组织带来了在类似项目中工作的调查人员， 技术结合在一起，从而降低成本，提高研究效率。

The Skin Phenotyping Core of the Skin Disease Research Center provides a wide array of histological, immunohistochemical, and molecular-based tools to characterize gross and microscopic morphology, functional features and gene expression in skin of mutant and genetically engineered laboratory animals. Special technical expertise within this core includes extensive knowledge of skin and hair follicle morphology essential for understanding the genotype/phenotype correlations; state of the art methods for gene expression studies at both mRNA and protein level specifically designed for mouse and human skin; and possession of a battery of original experimental approaches to the study of skin physiology in vivo. The Core will assist Center investigators as well as other researchers at Columbia in evaluating the significance of a phenotypic manifestation or unanticipated skin effects of a genetic mutation, drug, or other experimental manipulation; to understand the possible functions of genes and molecular pathways implicated in skin/hair phenotype development and to analyze skin expression patterns of genes of investigator's interest at the mRNA and protein level using immunohistochemistry and in situ hybridization. In addition, the Core will provide investigators with proof reading of and suggestions for Materials & Methods sections for publications and applications for extramural research funding utilizing skin phenotype analysis. The Skin Phenotyping Core also serves as a center of communication between investigators providing the ground for exchange of samples, antibodies, techniques and ideas. The Core brings investigators working in similar projects and techniques together thus reducing the costs and increasing efficiency of research.