FLOW CYTOMETRY RESOURCE

作为一个完全由癌症中心管理的机构，流式细胞术共享资源帮助癌症中心成员 基于流式细胞术的研究。服务包括： 荧光激活细胞分选（FACS） 免疫表型分析流式细胞术 ？流式细胞术用于分析细胞周期和凋亡 过去三年，由于收购了 新的工具。该设施现在运行五台仪器，包括两台荧光激活细胞 分选机，允许高速细胞分选，可以检测多达12个荧光参数， 先进的细胞分析仪，除了多色荧光，还能够执行特殊应用， 作为DMA含量的测定，多种（活体颜色）荧光蛋白分析和钙通量 测量.除了这些复杂的仪器，该设施还提供了两个用户操作的 分别允许3色或4色免疫分型以及细胞周期的基本细胞计数器 和凋亡测定。在过去的几年里，该设施的服务使130多万人受益。 调查人员，其中，仪器的主要使用者为CNOCC成员，约占60%。 高使用量使该设施能够以低费用提供服务， 委员会成员。该设施中的先进设备使哥伦比亚的调查人员能够 在流式细胞术领域进行最先进的研究，因此代表了一个重要的资源， 委员会成员。未来，我们计划提升流式细胞仪的功能，以方便检测和分选 新开发的活体彩色荧光蛋白，并购买仪器，作为一个灵活的 用于其他基于荧光的技术的分析平台，包括细胞计数珠测定（CBA）， 测量细胞因子的产生，以及其他潜在的应用。 在CCSG的最后一个阶段，使用该设施的研究者中有36%是癌症中心 拥有同行评审资金的成员，这些成员占使用量的50%至66%。 3可用服务该设施的拟议业务预算总额为425 004美元，其中我们是 向CCSG申请116，271美元

A fully Cancer Center-managed facility, the Flow Cytometry Shared Resource assists HICCC members in flow cytometry-based studies. Services include: ¿ Fluorescence-activated cell sorting (FACS) ¿ Analytical flow cytometry for immunophenotyping ¿ Flow cytometry for analyzing cell cycle and apoptosis The capacity of the facility has increased substantially over the past three years due to the acquisition of new instruments. The facility now operates five instruments, including two fluorescence-activated cell sorters that allow high-speed cell sorting and can detect up to 12 fluorescent parameters, and one advanced cell analyzer that besides multi-color fluorescence is able to perform specialty applications such as determination of DMA content, multiple (living color) fluorescent protein analysis and calcium flux measurements. In addition to those sophisticated instruments, the facility provides access to two useroperated basic cytometers permitting 3 or 4 color immunophenotyping, respectively, as well as cell cycle and apoptosis assays. Over the last several years, the services of the facility have benefited 130 CUMC investigators, with HICCC members comprising the main users of the instruments accounting for ~60%. The high usage volume allows the facility to offer the services at low fees, which are further reduced for HICCC members. The sophisticated equipment available in the facility allows investigators at Columbia to perform state-of-the-art research in the area of flow cytometry, and therefore represent a vital resource for HICCC members. In the future, we plan to upgrade the flow cytometers to facilitate detection and sorting of newly developed living color fluorescent proteins and to purchase an instrument that serves as a flexible analysis platform for additional fluorescence-based techniques, including cytometric bead assays (CBA) to measure cytokine production, among other potential applications. During the last period of the CCSG, 36% of the investigators using the facility were Cancer Center members with peer-reviewed funding, with those members representing from 50% to 66% of the usage of the 3 available services. The proposed total operating budget of the facility is $425,004, of which we are requesting $ 116,271 from the CCSG