Integrative Program in CNS Pathophysiology Research

这项提议的总体目标是将我们现有的Cobre第二阶段研究计划和核心设施转变为一个国家认可的研究中心，可以竞争Cobre独立的NIH中心拨款。在Cobre第一阶段，我们成功地开发了中枢神经系统病理生理学研究的综合计划，并利用NCRR的C06拨款建造了一个名为生物医学研究和综合神经成像(脑成像)中心的11,000平方英尺的专用综合神经成像设施。在第二阶段，我们升级了我们最先进的多模式神经成像核心设施，并将这些设施作为成功指导我们初级PI的基础设施。在第一和第二阶段，科布雷的9个初级私人投资机构中有6个成功地获得了总共9个新的NIH R01赠款。我们的同行评议出版物和赠款数量也大幅增加。为了支持这一应用，我们已经获得了约500,000美元的机构支持，以将我们的磁共振扫描仪升级为相控阵系统，并获得了额外的约400,000美元，以支持第三阶段计划的管理。申请中申请的资金将用于支持核心设施(三个核心-磁共振成像(MRI)、电子顺磁共振成像(EPRI)和具有双光子激光扫描显微镜的光学/电生理核心-的人员和运营成本)和试点项目计划(PPP)。核心设施和购买力平价的支持对于使我们的研究项目在国家层面上具有竞争力至关重要。在过去的8年里，我们的研究计划已经演变成四个不同的主题：i)血脑屏障中断的分子机制；ii)脑氧合及其作为可行的临床治疗的潜力；iii)干细胞和神经再生；以及iv)神经元损伤的分子机制。我们的研究战略是在中枢神经系统病理生理学的保护伞下建立一个连贯的、协同的研究计划，由这一应用的PI领导，他将与这四个主题的小组负责人合作。拟议的第三阶段Cobre的具体目标是：(1)维护和扩大全面的神经成像核心设施，以支持在新墨西哥州大学进行中枢神经系统病理生理学领域的基础研究和转化性研究；(2)通过加强对神经成像研究的关注，并通过拟议的总体战略规划和PPP增加NIH资助的研究人员的数量，维持和扩大协作和多学科的研究环境。

The overall goal of this proposal is to transition our existing Phase II COBRE research program and core facilities into a nationally recognized research center that can compete for COBRE-independent NIH center grants. During COBRE Phase I, we succeeded in developing an integrated program in CNS pathophysiology research, and built a 11,000 ft2 dedicated comprehensive neuroimaging facility called Biomedical Research and Integrative Neuroimaging (BRaIN Imaging) Center with a C06 grant from the NCRR. During Phase II, we have upgraded our state-of-the-art multimodal neuroimaging core facilities and used the facilities as the infrastructure for successfully mentoring our junior PIs. During Phase I and II, 6 out of 9 junior PIs in the COBRE successfully obtained a total of 9 new NIH R01 grants. Our peer-reviewed publications and number of grants also increased significantly. In support of this application we have obtained institutional support of ~$500,000 to upgrade our MR scanner into a phase array system and additional ~$400,000 to support the administration of the Phase III program. The funds requested in this application will be used support the Core facilities (personnel and operating costs of three cores - Magnetic Resonance Imaging (MRI), Electron Paramagnetic Resonance Imaging (EPRI) and Optical / Electrophysiology core that has a 2-photon laser scanning microscope) and a Pilot Project Program (PPP). The support of the core facilities and PPP is crucial for bringing our research program to a level competitive at the national level. During the last 8 years, our research program has evolved into four distinct topics: i) Molecular mechanism of BBB disruption; ii) Cerebral oxygenation and its potential as a viable clinical therapy; iii) Stem cells and neuroregeneration; and iv) Molecular mechanisms of neuronal injury. Our research strategy is to build a coherent, synergistic research program under the umbrella of CNS pathophysiology led by the PI of this application who will work with the group leaders of these four topics. The specific aims of the proposed Phase III COBRE are to: (1) maintain and expand the comprehensive neuroimaging core facilities that will support the conduct of basic and translational research in the area of CNS pathophysiology at UNM; and (2) sustain and expand a collaborative and multidisciplinary research environment by enhancing the focus on neuroimaging research and increasing the number of NIH-funded investigators through the proposed overall strategic planning and PPP.