SampleJet Dual Mode Automated Sample Changer with a Liquid Handler 215 and Temper

描述(由申请人提供)：一组由美国国立卫生研究院资助的研究人员申请资金，为现有的700 MHz核磁共振(核磁共振)光谱仪购买双模自动化、温度控制的自动取样器。这种自动进样器的加入对明尼苏达大学生物医学核磁共振设施中的许多大型临床代谢组学研究至关重要。新仪器将以自动化样品制备和高场核磁共振冷藏高通量能力的形式为大学研究人员提供许多好处。代谢组学是研究和分析生物体液中的代谢物，如血清、尿液和组织提取物，以了解疾病中的代谢途径，通常通过核磁共振和/或质谱分析。目前在明尼苏达大学提供的其他核磁共振自动进样器配备了用于在较低现场仪器上进行有机化学工作的设备，并且没有对自动进样器架上等待的样品进行温度控制的能力。能够冷却排队等待的核磁共振样品是我们要求最苛刻的研究项目的要求。在该区域的任何地方都没有这种性质的仪器，运输样品可能会对一些需要及时分析的样品造成损害。人血清和猪尿样本在室温下，在标准自动进样器(Low，Beilman)中等待不到72分钟即可显示降解。这一特性严重限制了对许多生物样本和不稳定代谢物(Peterson，Lim)进行大型研究的能力。由于缺乏适用于血清、尿液、唾液和组织提取液等液体的核磁共振自动化，明尼苏达大学的其他代谢组学研究已经主要局限于质谱学研究(纳尔斯图恩和彼得森)。在核磁共振设施进行的尖端研究在癌症、艾滋病、心脏和肾脏疾病、肌肉营养不良、瘫痪、糖尿病、中风、传染病、药物发现、骨骼疾病和阿尔茨海默氏症等领域拥有先进的发现和治疗。生物医学核磁共振设施的使命是提供高端核磁共振仪器和研究支持。用于临床研究的具有温度控制的高通量自动取样器是实现其支持明尼苏达大学健康研究的使命的关键下一步。 与公共卫生相关：这项提案要求为一台高灵敏度700 MHz核磁共振仪器配备一个高通量、带冷藏样品架的机器人自动取样器，为明尼苏达大学的研究人员提供扫描大量生物样品的实验能力。在该仪器的支持下，这项研究将允许对包括血清、尿液、脑脊液和组织提取物在内的样本进行自动化制备、适当的温度处理和分析。对该仪器的研究可以为新陈代谢差异提供新的见解，并有可能开发新的疾病和状况诊断测试，包括癌症、失血性休克、艾滋病、心脏病、肌肉营养不良、瘫痪、糖尿病、中风、传染病、药物发现、骨骼疾病和阿尔茨海默氏症。

DESCRIPTION (provided by applicant): A group of NIH-funded investigators request funds to acquire a dual-mode automated, temperature-controlled autosampler for an existing 700 MHz nuclear magnetic resonance (NMR) spectrometer. The addition of this autosampler instrument is critical to many large clinical metabolomic research studies in the Biomedical NMR facility at the University of Minnesota. The new instrument will provide numerous benefits to University researchers in the form of automated sample preparation and refrigerated high-throughput capability with high-field NMR. Metabolomics is the study and analysis of metabolites in biofluids such as serum, urine and tissue extracts to understand metabolic pathways in disease typically through NMR and/or mass spectrometry analysis. Other NMR autosamplers currently available at the U of Minnesota are equipped for organic chemistry work on lower field instruments and have no temperature control capability for samples waiting in the autosampler rack. The ability to cool NMR samples waiting in a queue is a requirement for our most demanding research projects. An instrument of this nature is not available anywhere in the regional area and transporting the samples could be detrimental to some samples that need timely analysis. Human serum and porcine urine samples can show degradation at room temperature in less than 72 minutes waiting in a standard autosampler (Low, Beilman). This property severely limits the ability to conduct large studies with many biological samples and for unstable metabolites (Peterson, Lim). Other metabolomic research at the U of Minnesota has already been mostly restricted to mass spectrometry studies (Nelsestuen and Peterson) due to the lack of NMR automation appropriate for fluids like serum, urine, saliva, and tissue extracts. The cutting edge research conducted in the NMR facility has advanced discoveries and treatments in the areas of cancer, AIDS, heart and kidney disease, muscular dystrophy, paralysis, diabetes, stroke, infectious disease, drug discovery, bone disease, and Alzheimer's. The mission of the Biomedical NMR facility is to provide high-end NMR instrumentation and research support. A high throughput autosampler with temperature control for clinical studies is a critical next step in fulfilling its mission to support health research at the University of Minnesota. PUBLIC HEALTH RELEVANCE: This proposal requests a high-throughput, robotic autosampler with a refrigerated sample rack for a high sensitivity 700 MHz nuclear magnetic resonance instrument to provide the experimental capability to scan large numbers of biological samples to researchers at the University of Minnesota. The research supported by this instrument addition will allow the automated preparation, appropriate temperature handling, and analysis of samples including serum, urine, cerebral spinal fluid, and tissue extracts. Research on this instrument can provide new insights into metabolic differences and potentially develop new diagnostic tests for diseases and conditions including cancer, hemorrhagic shock, AIDS, heart disease, muscular dystrophy, paralysis, diabetes, stroke, infectious disease, drug discovery, bone disease, and Alzheimer's.