Characterization and Synthesis of Conopeptides

该核心将开展五项主要和独特的活动：肽合成，肽分析和表征，合成和天然肽的质谱分析，合成和分析挑战和发现项目所需的方法学开发。 将使用固相方法和Boc或Fmoc策略进行肽合成。 肽分析和表征将使用HPLC、CZE、CD和MS。 方法学的发展可能包括合成新的氨基酸或测试新的色谱支持，等等。 将独立使用质谱法，通过在犹他州大学和索尔克研究所进行的纯化步骤跟踪天然芋螺毒素。 发现项目将集中在设计新的类似物的选定的毒素使用氨基甘氨酸和norcysteine支架除了丙氨酸扫描。 这个核心，通过汇集一个既定小组的综合和分析专业知识，将使该计划项目的成员能够经济和及时地实现各自的目标。 计划项目主任和本核心项目的主要研究者确定优先事项并定期沟通。日常操作将由J. Rivier博士和W.索尔克研究所的费舍尔和M.犹他州大学的麦金托什。

This Core will carry out five main and distinct activities: peptide synthesis, peptide analysis and characterization, mass spectrometric analysis of synthetic and native peptides, methodological developments as required by synthetic and analytical challenges and discovery projects. Peptide synthesis will be done using the solid phase approach and either Boc or Fmoc strategies. Peptide analysis and characterization will use HPLC, CZE, CD and MS. Methodological developments may include the synthesis of novel amino acids or the testing of novel supports for chromatography, among others. Mass spectrometry will be used independently to follow native conotoxins through their purification steps carried out at the University of Utah and the Salk Institute. Discovery projects will concentrate on the design of novel analogs of selected toxins using the aminoglycine and norcysteine scaffolds in addition to Ala-scans. This Core, by bringing together the synthetic and analytical expertise of an established group will enable members of this Program Project to reach their respective goals both economically and in a timely fashion. The Program Project Director and the PI of this Core set priorities and communicate regularly. Day to day operations will be coordinated by Drs. J. Rivier and W. Fischer at the Salk Institute and Dr. M. Mclntosh at the University of Utah.