EVALUATION OF ALTERNATIVE METHODS FOR MEASURING HUMAN SALIVARY AMYLASE ACTIVI

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 Roche Diagnostics/Hitachi 911总淀粉酶活性测定法适用于唾液淀粉酶活性测定。 Thermo Appliskan酶标仪是Dynatech Lab MRX酶标仪的合适替代品，可采用Salimetrics方法测定唾液淀粉酶活性。 我们的第一个具体目标是比较Roche Diagnostics/Hitachi 911方法的总淀粉酶活性与Salimetrics方法的唾液淀粉酶活性，使用Dynatech Lab MRX酶标仪，使用合并的唾液和单个唾液标本，以获得准确度，精密度，动态范围和方法之间的一致性。 我们的第二个具体目标是比较Thermo Appliskan酶标仪与Dynatech Lab MRX酶标仪的性能，采用Salimetrics方法测定唾液淀粉酶活性，使用合并唾液和个体唾液标本，以获得准确度、精密度、动态范围和方法间的一致性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Roche Diagnostics/Hitachi 911 method for total-amylase activity is suitable for the measurement of salivary amylase activity. The Thermo Appliskan microplate reader is a suitable substitute for the Dynatech Lab MRX microplate reader with the Salimetrics method for salivary amylase activity. Our first specific aim is to compare the Roche Diagnostics/Hitachi 911 method for total-amylase activity to the Salimetrics method for salivary amylase activity utilizing the Dynatech Lab MRX microplate reader, with both pooled saliva and individual saliva specimens, for accuracy, precision, dynamic range, and agreement between methods. Our second specific aim is to compare the performance of the Thermo Appliskan microplate reader to the performance of the Dynatech Lab MRX microplate reader with the Salimetrics method for salivary amylase activity, utilizing both pooled saliva and individual saliva specimens, for accuracy, precision, dynamic range, and agreement between methods.