INBRE-2 HIGH-THROUGHPUT SEQUENCING OF SELF-INCOMPATIBILITY ALLELES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 本研究旨在探讨罂粟属植物自交不亲和性基因座(S基因座)的变异规律。自交不亲和性(SI)是一种允许植物避免自交受精的遗传系统。自我识别基因座，如植物中的自交不亲和基因座，具有惊人的多样性，既有大量功能不同的等位基因，也有由于负频率依赖选择而导致的等位基因之间的严重差异。我们以前的工作是用简并的聚合酶链式反应发现新的S等位基因。然而，这种方法一直是低效的，而且可能会在我们迄今发现的等位基因中引入偏见，这是由于S基因座不同等位基因之间序列保守性较低。为了准确了解该基因座的分子群体遗传多样性，需要一种更有效、更少偏见的方法。我们建议测试下一代测序技术是否可以用于更好地发现新的S基因座等位基因。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project is designed to investigate the diversification pattern of the self-incompatibility locus (S-locus) in the poppy genus, apaver. Self-incompatibility (SI) is a genetic system that allows plants to avoid self-fertilization. Self-recognition loci, such as the self-incompatibility loci in plants are stunningly diverse, with both large numbers of functionally different alleles, and deep divergence among alleles as a result of negative frequency dependent selection. Our previous work has used degenerate PCR to discover new S-alleles. However, this approach has been inefficient and has likely introduced bias in the alleles that we have discovered to date due to low sequence conservation among different alleles in the S-locus. A more efficient, less biased approach is required for an accurate picture of molecular population genetic diversity at this locus. We propose to test whether next-generation sequencing (NGS) technology can be employed for improved discovery of new S-locus alleles.