Analytical Core/Richard B. van Breemen

该分析核心将提供基本的质谱学(MS)和核磁共振 (核磁共振)测量，以支持植物中心的所有项目和其他核心。这些 衡量标准将包括常规分析研究以及新分析方法的开发。 以支持中心内不断发展的研究。分析核心人员将为 为中心内的所有核心和项目设计和解释分析测量。在.期间 植物学中心的前10年，这个核心的分析测量是基于质量 包括高分辨率准确的质量测量，液相色谱-质量联用 光谱(LC-MS)、串联质谱仪(MS-MS)、LC-MS-MS和脉冲超滤LC-MS(a 本实验室发明的快速识别药理靶标配基的技术)。除了……之外 提供这些基于质谱学的分析技术，这个核心的分析能力将是 扩展到包括常规的质子和碳13 ID和2D核磁共振以及使用600、800和 900 MHz仪器。因此，这一核心将能够提供更完整的分析范围 测量。在之前的资助期间，已经在项目中开发了几种分析分析方法 期间，该核心将在下一年向所有项目和核心提供这些新的分析服务 资助期。将提供的一些测量示例包括高分辨率1D和2D 核磁共振，用于确定元素组成的高分辨率准确质量LC-MS测量， 支持项目1、项目3、核心B和 核心C和超滤-LC-MS筛选分析。根据需要，新的MS和核磁共振方法将 开发并实现了对植物天然产物的检测、表征和定量分析 植物提取物和生物液体中的产品及其代谢物。总而言之，该核心将提供 MS和核磁共振测量和分析专业知识，以提高所有项目和岩心的生产率 中心。

This Analytical core will be to provide essential mass spectrometry (MS) and nuclear magnetic resonance (NMR) measurements in support of all Projects and other Cores of the Botanical Center. These measurements will include routine analytical studies as well as the development of new analytical methods to support evolving research within the Center. Analytical Core personnel will provide expertise for the design and interpretation of analytical measurements for all Cores and Projects within the Center. During the first 10 years of the Botanical Center, the analytical measurements withing this core were based on mass spectrometry and have included high resolution accurate mass measurements, liquid chromatography-mass spectrometry (LC-MS), tandem mass spectrometry (MS-MS), LC-MS-MS, and pulsed ultrafiltration LC-MS (a technique invented in this laboratory for rapidly identifying ligands to pharmacological targets). In addition to providing these mass spectrometry-based analytical techniques, the analytical capabilities of this core will be expanded to include routine proton and carbon 13 ID and 2D NMR and high field NMR using 600, 800 and 900 MHz instruments. Therefore, this core will be able to provide a more complete range of analytical measurements. Several analytical assays have been developed in the projects during the previous funding period, and this core will provide these new assays as services to all Projects and Cores during the next funding period. Examples of some measurements that will be provided include high resolution 1D and 2D NMR, high resolution accurate mass LC-MS measurements for the determination of elemental compositions, MS-MS fragmentation pattern studies in support of structure elucidation in Project 1, Project 3, Core B, and Core C, and ultrafiltration-LC-MS screening assays. As needed, new MS and NMR methods will be developed and implemented for the detection, characterization and quantitative analysis of botanical natural products and their metabolites in botanical extracts and biological fiuids. In summary, this core will provide MS and NMR measurements and analytical expertise to enhance the productivity of all Projects and Cores in the Center.