MRI OF INSECT FLIGHT MUSCLE IN A BEETLE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 车身长度车身宽度车身厚度 男性 70毫米20毫米15毫米 女性 70-75毫米20-22毫米 1)一只大型甲虫胸部（包括MRI中的腹部）飞行肌肉的三维内部结构图像，这只甲虫是一只强壮的飞行器（刚刚从亚利桑那州图森市收集和联邦快递的帕洛韦尔迪root borers（Derobrachus geminatus））。 雄性甲虫的身体大小为70毫米长，20毫米宽，15毫米厚。 如果不容易塞到死甲虫的尸体旁边，腿和触角可以被修剪或移除。 我想要核磁共振成像，以便有一个在原位的三维视图的飞行肌肉不受解剖。 100 um分辨率就可以了。 10微米可能是有趣的，如果avsilable和100微米已经预订了。甲虫是如此严重的硬化，温和的飞行肌肉解剖是非常困难的;我从来没有成功与随机捕捉当地数控甲虫。 甲虫是最后的4个家庭的飞行昆虫的共同类型的飞行肌肉，我想研究电子显微镜和纤维x射线衍射。在开始核磁共振前我会用HCN或氮气窒息甲虫。只有幼虫吃。 雄性甲虫在孵化后并不进食，而是四处飞行寻找雌性甲虫。 我还不知道它们单靠水能活多久，也许不到一周。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Body length Body width Body thickness Male 70 mm 20 mm 15 mm Female 70-75 mm 20-22 mm 1) Image 3D internal architecture of flight muscle in thorax (and include abdomen in MRI) of a large beetle known to be a strong flyer (palo verde root borers (Derobrachus geminatus) just collected and fedexed from Tucson, Arizona). The size of the male beetle's body is 70 mm long, 20 mm wide and 15 mm thick. Legs and antennae can be trimmed or removed if not easily tucked in alongside the body of a dead beetle. I want the MRI in order to have an in situ 3D view of the flight muscle undisturbed by dissection. 100 um resolution is ok. 10 um might be interesting if avsilable and 100 um already booked up. Beetles are so heavily sclerotized that gentle flight muscle dissection is very difficult; I have never suceeded with random-catch local NC beetles. Beetles are the last of 4 families of flying insects whose shared type of flight muscle I want to study by electron microscopy and fiber x-ray diffraction. I will asphyxiate a beetle with HCN or nitrogen gas just before starting MRI. Only the larvae eat. The male beetles are not known to eat after hatching and flying about to find and impregnate females. I don't know yet how long they can live on water alone, maybe less than a week.