STRUCTURAL STUDIES OF THE EXOMER COAT PROTEIN COMPLEX

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 三分之一的基因产物被认为是穿梭于真核细胞的分泌系统中。有许多人类疾病与分泌系统组件的缺陷有关，或者与破坏蛋白质与膜运输机制接口的能力的突变有关。蛋白质在膜结合的隔室之间的转运是一个涉及囊膜载体的严格调控的过程。一个经典的膜泡是通过膜变形而产生的，部分是由胞质外壳蛋白实现的。外壳蛋白不仅形成膜，而且在选择纳入运输载体的货物时起着重要作用。最近的研究证实，酵母中存在一种新的膜包膜复合体，负责将精选的货物从反式高尔基体网络(TGN)运输到质膜，绕过内体系统。我们已经结晶了外聚体外壳的一个亚复合体，并希望确定它的结构，作为了解它如何发挥作用的一步。在这一点上，我们的晶体很小，我们还没有在家用x射线源上测试它们的衍射，我们相信需要棋子光束时间来收集有意义的数据。我们将用我们的象棋波束时间来筛选我们的各种晶体形态和低温保护条件，幸运的是，我们将收集完整的本地数据集。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A full third of all gene products are thought to be shuttled through the secretory system of eukaryotic cells. There are numerous human diseases associated with defects in components of the secretory system, or with mutations that disrupt the ability of a protein to interface with the membrane trafficking machinery. Transport of protein ¿¿Scargo¿¿¿ between membrane-bound compartments is a tightly regulated process involving vesicular membrane carriers. A classical membrane vesicle is created via membrane deformation achieved in part by cytosolic ¿¿Scoat¿¿¿ proteins. Coat proteins not only shape membranes, but also play an important role in selecting cargo incorporated into transport carriers. Recent studies have established the existence of a new membrane coat complex responsible for trafficking select cargo from the trans-Golgi network (TGN) to the plasma membrane in yeast, bypassing the endosomal system. We have crystallized a subcomplex of the exomer coat and hope to determine its structure as a step towards understanding how it functions. At this point, our crystals are small and we have not yet tested their diffraction on a home x-ray source, and we believe CHESS beamtime will be required to collect meaningful data. We will use our CHESS beamtime to screen through our various crystal forms and cryo-protectant conditions, and with luck we will be collecting full native datasets.