EM STUDY OF THE AUTOPHAGY PATHWAY AFTER BRAIN ISCHEMIA

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 本研究的目的是研究短暂性脑缺血再灌流后自噬通路的损伤。我们的方法将依靠光学和电子显微镜相结合的方法来评估缺血对自噬途径和相关蛋白的影响。特别是广域电子断层扫描的使用，应该会显着提高我们检查组织样本的效率，从而增加这项研究的力量。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The objective of this proposal is to investigate impairment of the autophagy pathway after transient cerebral ischemia followed by reperfusion. Our approach will rely on a combination light and electron microscopy to assess the effect of ischemia on the autophagy pathway and associated proteins. The use of wide-field electron tomography in particular should significantly increase the efficiency with which we can examine tissue samples and thereby increase the power of this study.