ENERGY DISPERSION OF PROTEIN LAUE DIFFRACTION SPOTS
ENERGY DISPERSION OF PROTEIN LAUE DIFFRACTION SPOTS
批准号:
8171974
负责人:
Timothy J. Graber
金额:
$4.01万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2010
资助国家:
美国
项目状态:
已结题
起止时间:
2010-08-01 至 2011-07-31
关键词:
中文摘要
这个子项目是许多研究子项目中的一个
由NIH/NCRR资助的中心赠款提供的资源。子项目和
研究者(PI)可能从另一个NIH来源获得了主要资金,
因此可以在其他CRISP条目中表示。所列机构为
研究中心,而研究中心不一定是研究者的研究机构。
在衍射实验中,每一组平面(hkl)与间距d在单晶作为一个单色器,必须满足布拉格?s定律。 因此,每一组平面都会产生一个单独的平面。反思?在具有由晶体无序、晶体和光束的有限尺寸等限定的扩展的衍射图案中,和光束的光谱特性。对于多色X射线束(粉红色光束),劳厄衍射图中的晶体出现斑点。在拟议的实验中,我们将测量粉红色光束的单个脉冲的能量色散的小分子/蛋白质晶体,并将其与波荡器的光谱特性进行比较。将使用放置在晶体衍射仪之后的Si单晶分析仪研究晶体(hkl)平面反射的X射线束的能量分布。
英文摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
In a diffraction experiment each set of planes (hkl) with spacing d in the single crystal acts as a monochromator that must satisfy Bragg?s Law. Each set of planes thus gives rise to a single ?reflection? (spot) in the diffraction pattern with extensions which is defined by a crystal disorder, finite dimensions of the crystal and the beam etc., and spectral characteristics of the beam. For polychromatic X-Ray beam (pink beam) spots occur from crystal in Laue diffraction pattern. In proposed experiment we shall measure energy dispersion of a single pulse of the pink beam by a small molecule/ protein crystal and compare it to the spectral characteristics of the undulator. The energy profile of the X-Ray beam reflected by (hkl) plane of the crystal will be studied using Si single crystal analyzer placed after the crystal diffractor.
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