BIODEGRADATION BY WHITE ROT PHANEROCHATE CHRYSOSPORIUM

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 纤维素生物质是植物细胞壁中不可食用的纤维部分，具有巨大的潜力，可以作为糖类的来源，作为平台化学品来制造燃料、材料和其他化学品。然而，生物质的复杂结构和组成阻碍了其有效的降解和转化为糖。大多数糖被包裹在半纤维素和木质素包裹的结晶纤维素纤维中。我们正指望大自然来克服这一技术障碍。虽然许多真菌会降解部分生物质，但白腐菌在完全降解生物质方面是独一无二的。通过不同技术的组合，我们希望捕捉白腐真菌Phanerochaete chrysosporium在杨树(一种具有商业潜力的生物质资源)上的降解策略，并为可能的工业应用进行优化。我们的主要科学目标是利用质谱仪确定黄孢霉在降解过程中分泌的关键代谢物和酶，然后将这些因素与杨树样品中的特定降解事件联系起来。我们希望通过对真菌降解不同阶段的杨树样品进行仔细的结构和成分分析来确定这些特定的降解事件。我们正在使用使用BioCAT Beamline 18ID的扫描微探针X射线衍射(SMX)作为我们解决这个问题的工具之一，我们希望这项工作不仅将促进我们对对我们的生态系统具有根本重要性的生物过程的理解，而且它还将提供指导工业设计的见解

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cellulosic biomass, the inedible fibrous part of plant cell walls, has great potential as a source of sugars that could be used as platform chemicals to make fuels, materials and other chemicals. However, the complex structure and composition of biomass impedes its efficient degradation and conversion into sugars. Most sugars are locked in crystalline cellulose fibers which are sheathed in hemicellulose and encrusted in lignin. We are looking to nature to overcome this technical roadblock. Although many fungi degrade parts of biomass, white rot fungi are unique in their ability to degrade it completely. By using a combination of different techniques we hope to capture the degradation strategies of white rot fungus Phanerochaete chrysosporium on Poplar (a biomass source with commercial potential) and then to optimize for possible industrial application. Our major scientific objectives are to define the key metabolites and enzymes secreted by P.chrysosporium during degradation, using mass spectrometry, and then to relate these factors to specific degradation events in the Poplar samples. We hope to identify those specific degradation events by careful structural and compositional analysis of Poplar samples at different stages of fungal degradation. We are using scanning microprobe X-ray diffraction (SMX) using the BioCAT beamline 18ID as one of our tools to approach this problem, Our hope is that not only will this work advance our understanding of a biological process of fundamental importance to our ecosystem but that it will also provide insights that guide the design for industrial