Large Animal Core

项目总结(见说明)： 大型动物核心中心将提供所需的设施、管理和技术专长，为拟议计划的所有项目提供MGH小型猪和狒狒和组织样本，以及专家兽医护理、免疫试剂和微生物学诊断分析和支持。临床样本将被分发，以最大限度地利用每种动物，以研究临床相关的异种移植方案。该计划的每个组成部分的成功取决于该核心提供的动物、微生物支持和抗体的质量、可预测性和可获得性。 动物护理每周在主要调查人员和兽医工作人员的会议上进行审查。 大型动物核心的目标包括： 目的1：MGH MHC近交系小型猪的生产、质量控制、跟踪和维护，包括新型MGH GALT-KO猪、α-1，3-半乳糖基转移酶基因纯合缺失(见图1和2)和GALT-KO-CD39转基因猪。小型猪的生产是使用一个计算机化的、基于网络的系统来监控的，该系统的开发是为了协调每个项目的动物的饲养、质量控制、分配、运输和利用。生猪生产的显著改善使猪的供应量增加，足以满足拟议的研究。 目标2：购买和维护非人类灵长类动物 目的3：为免疫缺陷动物提供传染病/微生物学支持，为病毒病原体提供分子诊断分析，并为传染病管理提供建议。 目的4：制备、鉴定和维持一系列独特的与猪和狒狒细胞表面抗原反应的单抗，以评估猪和狒狒细胞的谱系和改善细胞的耗竭。 这些活动的协调由Scott Arn负责，他负责基因敲除猪的发育、灵长类动物的提供和MGH移植生物学研究中心所有研究人员使用的详细数据库的维护。杰伊·菲什曼博士将负责异种移植研究中的传染病管理和微生物学诊断。动物手术由经验丰富的移植外科医生、兽医临床医生和麻醉师在专门的无菌手术室进行，以优化护理和舒适度。

PROJECT SUMMARY (See instructions): The Large Animal Core will provide the facilities, management and technical expertise needed to supply MGH miniature swine and baboons and tissue samples for all projects of the proposed program as well as expert veterinary care, immunological reagents, and microbiologic diagnostic assays and support. Clinical samples will be distributed to maximize utilization of each animal for studies of clinically relevant regimens for xenotransplantation. The success of each component of this program is dependent on the quality, predictability, and accessibility of the animals, microbiologic support, and antibodies provided by this Core. Animal care is reviewed weekly at meetings of the principal investigators and the veterinary staff. The Aims of the Large Animal Core include: Aim 1: Production, quality control, tracking, and maintenance of MGH MHC inbred miniature swine including the novel MGH GalT-KO swine, with homozygous disruption of the a-1,3-galactosyltransferase gene (see Figures 1 and 2) and the GalT-KO-CD39 transgenic swine. Miniature swine production is monitored using a computerized, web-based system developed to coordinate the breeding, quality control, allocation, transportation and utilization of animals for each Project. Significant improvements in swine production have achieved an increased supply of swine adequate to the proposed studies. Aim 2: Purchase and maintenance of non-human primates Aim 3: Infectious Disease/Microbiology support for immunocompromised animals to provide molecular diagnostic assays for viral pathogens and advice on infectious disease management. Aim 4: Production, characterization, and maintenance of a unique series of monoclonal antibodies (MAb)reactive with swine and baboon cell surtace antigens to assess pig and baboon cell lineages and improve cellular depletion. Coordination of these activities is performed by Scott Arn, who directs all aspects of the development of the knockout swine, the provision of primates and maintenance of the detailed database used by all investigators of the Transplantation Biology Research Center at MGH. Dr. Jay Fishman will oversee infectious disease management and microbiologic diagnostics in xenotransplantation studies. Animal procedures are conducted by experienced transplant surgeons, veterinary clinicians and anesthesiologists in dedicated sterile operating rooms to optimize care and comfort.