Colitis and Colitis Associate Cancer Core

该计划的总体目标是了解慢性炎症如何导致肿瘤 晋升和进步。目前的计划将涉及伤害/病原体引发的几个方面 聚焦于TLRs的信号通路，参与转录和转录后改变 控制，以及炎症信号和肿瘤之间的因果联系。链接的主要组件 其中三个项目涉及更广泛地利用小鼠遗传学和小鼠模型 炎症性疾病和癌变。在项目1、3和4中的每个项目中，都将有新的转基因小鼠 在几个体内炎症反应模型中产生、表征和测试。的一个中心焦点 这些研究将利用DSS结肠炎的小鼠模型及其与结肠肿瘤发生的联系。 这一中心活动将由新提出的核心B(小鼠结肠炎和结肠炎相关)协调 癌症分析核心)。此核心的结肠炎分析部分的主要目标是设置结肠炎 由DSS、TNBS或领养转移诱导的模型，并在分析 项目产生的转基因小鼠的结肠炎症状、体内稳态和炎症状态。 这个核心的结肠炎相关癌症部分的主要目标是建立AOM+DSS行业的CAC 在表型分析(包括肿瘤数量、肿瘤大小、 组织学分析和其他分析)。标准化的方案、评价和定量分析 结肠炎和肿瘤发生将促进实验室间的合作和数据比较。 相关性(请参阅说明)： 这项活动将标准化结肠炎和结肠炎的方案、评估和量化分析- 相关的癌症模型，以便在各个项目中获得一致的结果。

The overall objective of the Program is to understand how chronic inflammation contributes to tumor promotion and progression. The current program will address several aspects of injury/pathogen-initiated signaling pathways focusing upon TLRs, the involvement of altered transcriptional and post-transcriptional control, and the causal links between inflammatory signaling and neoplasia. A major component that links three of the projects involves much expanded utilization of mouse genetics and mouse models of inflammatory disease and carcinogenesis. In each of project 1, 3 and 4, new transgenic mice will be generated, characterized and tested in several models of in vivo inflammatory response. A central focus of these studies will be the use of the mouse model of DSS colitis and its linkage with colon tumorigenesis. This central activity will be coordinated by the newly propose Core B (Mouse Colitis and Colitis-associated Cancer Analysis Core). The colitis analysis portion ofthis Core's primary objective is to set up a colitis model induced by DSS, TNBS or adoptive transfer and provide technical assistance in the analysis of the colitis symptoms, states of homeostasis and inflammation induced in transgenic mice produced by projects. The colitis-associated cancer portion ofthis core's primary objective is to set up the AOM+DSS-indueed CAC model and provide specific technical assistance in phenotype analyses (including tumor number, tumor size, histology analysis and other assays). Standardization of the protocols, evaluation and quantitative assays of colitis and tumorigenesis will facilitate inter-laboratory collaboration and data comparisons. RELEVANCE (See instmctions): This activity will standardize the protocols, evaluation and quantification assays for colitis and colitis- associated cancer models, so that consistent results will be obtained across the projects.