Mouse Core

老鼠核心将负责培育和提供项目所需的所有老鼠 计划项目。这三个项目对此核心的预期使用情况为：项目1(40%)、项目 2(30%)、项目3(30%)。这个小鼠核心将是瓦克兰小鼠群体(WMC)的一个组成部分， 这是一个SPF菌落，包含1500个笼子(每天约5000次普查)。这一核心有两个具体目标。这个 第一个具体目标将是保持PPG调查人员所需的所有各种小鼠品系，并 生产并提供供所有实验使用的小鼠。这将需要维护40多个 独特的老鼠品系。核心将负责维护繁殖记录，验证 所有B6同源菌株的基因分型，并产生所需的实验小鼠队列 项目1、2和3中的人员进行的实验。第二个具体目标将是生产和基因分型 所有项目将产生的新菌株。这将包括培育所有转基因创始人 菌株，并将选定的转基因创建者导入特定的基因类型。核心也是 负责培育老鼠并将其运送给生物医学研究界的其他研究人员 请求访问我们收藏的老鼠。

The mouse core will be responsible for breeding and providing all mice needed by the Projects within the Program project. The anticipated usage of this core by these three Projects will be: Project 1 (40%), Project 2 (30%), Project 3 (30%). This mouse core will be a component of the Wakeland mouse colony (WMC), which is an SPF colony containing >1500 cages (~5000 daily census). This core has two specific aims. The first specific aim will be to maintain all of the various mouse strains required by PPG investigators and to produce and provide mice for use in all experiments. This will require the maintenance of more than 40 unique strains of mice. The core will be responsible for maintaining breeding records, validating the genotypes of all the B6-congenic strains, and producing experimental cohorts of mice as needed for experiments by personnel in Projects 1, 2, and 3. The second specific aim will be to produce and genotype the new strains that will be produced by all of the projects. This will include breeding all transgene founder strains and introgressing the selected transgene founders onto specific genotypes. The core is also responsible for breeding and shipping mice to other investigators in the biomedical research community requesting access to the mice in our collection.