Biophysical Inaging Core

生物物理成像核心的主要目标是为项目调查人员提供完整的 一系列专业知识、培训、设备和数据分析工具，以获得纳米到微米尺度的生物物理 关于内皮屏障通透性的细胞和分子基础的信息。核心D 人员和设备将允许PPG科学家对3D结构进行成像，评估物理和化学 属性和定义微扰诱导的细胞结构和活性的实时变化 亚细胞成分，包括膜、细胞骨架网络、细胞-基质和细胞-细胞连接。 该生物成像核心支持PPG的五个研究项目，其定量显微镜与以下相关 原子力显微镜、光荧光显微镜和TIRF(全内反射 荧光显微镜)。它提供了对中心完整资源的有经验的使用途径 纳米医学，包括最先进的原子力显微镜，集成了高分辨率单 用于同时进行多模式相关研究的光子显微镜和荧光显微镜系统。在……里面 此外，这个核心还将利用芝加哥大学IBD提供的公共资源 (生物物理动力学研究所)显微镜设备(包括一台极好的电子显微镜 设备)和医学部的多光子激光扫描显微镜设备。由酷睿引领 领导Ratnesh Lai博士，核心已经集结了人员和实验室设施，以满足广泛 实验3D成像和机械生物物理学所需的范围。核心D人员具有专业性 在高分辨率成像和检测物理和化学性质领域的经验， 包括机械生物物理学和各种扫描探头显微镜。尽管相对最近到来的是 芝加哥大学的核心人员，这些才华横溢的科学家提供了令人信服的 每个项目和所有五个研究项目的初步数据。

The principal objective of the Biophysical Imaging Core is to provide Program investigators with a complete range of expertise, training, equipments, and data analysis tools to obtain nano-to-micro scale biophysical information pertaining to the cellular and molecular basis of endothelial barrier permeability. Core D personnel and equipment will allow PPG scientists to image 3D structures, evaluate physical and chemical properties and define perturbant-induced real-time changes in the structures and activity of cells and subcellular constituents, including membranes, cytoskeletal networks and cell-matrix and cell-cell junctions. This Biolmaging Core supports the PPG's five research projects with quantitative microscopy related to atomic force microscopy (AFM), light fluorescence microscopy and TIRF (total internal reflection fluorescence microscopy). It offers access to experienced use of the complete resources of the Center for Nanomedicine that includes state-of-the-art atomic force microscopes integrated with high resolution single photon microscopy and fluorescence microscopy systems for simultaneous multimodal correlative studies. In addition, this Core will also make use of the common resources available at the University of Chicago IBD (Institute for Biophysical Dynamics) Microscope Facility (which includes an excellent electron microscope facility) and the Department of Medicine's Multiphoton Laser Scanning Microscope Facility. Led by Core Leader Ratnesh Lai, PhD, the core has assembled the personnel and laboratory facilities to satisfy a wide range of experimental 3D imaging and mechanobiophysics needs. Core D personnel have professional experience spanning the fields of high resolution imaging and examining physical and chemical properties, including mechanobiophysics with various scanning probe microscopies. Despite relative recent arrival of Core personnel within the University of Chicago, these talented scientists have provided convincing preliminary data for each Project and all five research projects.