Mouse Genetic Models

小鼠遗传模型已被华盛顿大学肌肉骨骼研究所广泛使用 研究人员是肌肉骨骼生物学和医学研究的核心。尽管有 在肌肉骨骼研究中，个人研究人员进行了许多成功的研究活动 在社区中，没有一种机制来促进生产、获取和分享 在研究人员中发现了相关的小鼠遗传模型。也缺乏培训和培训 针对非专家用户的教育。因此，为了营造一个更充分和更协同的研究环境， 华盛顿大学的肌肉骨骼研究人员，核心致力于小鼠遗传模型 支持中心研究人员生产、保存和分享转基因小鼠 及时、可靠的方式。具体地说，小鼠遗传模型的核心是实现四个具体目标。 目的1：转基因小鼠的生产。小鼠基因组可以用两种常规方法改变 方法：(I)通过直接将DNA注入单细胞胚胎来整合合成基因，或(Ii) 同源重组在胚胎干细胞中诱导的靶向突变的建立 然后整合到生殖系中。对于ES细胞的操作，我们将利用现有的ES 科尔在华盛顿大学工作。对于正确靶向的ES细胞或DNA构建物的显微注射， 我们将利用现有的Mouse Genetics Core。目的：转基因小鼠品系的冷冻保存。这 也将在现有的Mouse Genetics Core执行。目的3：咨询和教育。《核心》 董事们将就老鼠的遗传策略向调查人员提供建议。目的4：维护可用小鼠的数据库 模特们。这一目标利用华盛顿大学现有的专业知识和试剂来 建立和宣传全校与肌肉骨骼相关的转基因小鼠品系数据库 学习。总体而言，小鼠遗传模型的核心将最大限度地利用现有的小鼠遗传 通过补贴服务提供资源，加快华盛顿大学的肌肉骨骼研究。

Mouse genetic models have been extensively utilized by Washington University musculoskeletal investigators and are central to research of musculoskeletal biology and medicine. Although there have been many successful research activities by individual investigators within the musculoskeletal research community, there has not been a mechanism in place to facilitate the production, acquisition and sharing of pertinent mouse genetic models among the investigators. There has also been a lack of training and education for non-expert users. Thus, to foster a more sufficient and synergistic research environment for musculoskeletal researchers at Washington University, the Mouse Genetic Models Core is dedicated to supporting production, preservation and sharing of genetically altered mice for Center investigators in a timely and reliable manner. Specifically, the Mouse Genetic Models Core is to achieve four specific goals. Aim1: Production of genetically modified mice. The mouse genome can be altered using two general approaches: (i) incorporation of synthetic genes via direct injection of DNA into single-celled embryos, or (ii) creation of targeted mutations that are induced in embryonic stem (ES) cells by homologous recombination and then incorporate into the germ line. For ES cell manipulation, we will take advantage of the existing ES core at Washington University. For microinjections of either correctly targeted ES cells or DNA constructs, we will utilize the existing Mouse Genetics Core. Aim2: Cryopreservation of transgenic mouse strains. This will also be performed at the existing Mouse Genetics Core. Aim3: Consultation and education. The Core Directors will advice investigators on mouse genetic strategies. Aim4: Maintain database of available murine models. This aim takes advantage of the existing expertise and reagents at Washington University to establish and publicize a campus-wide database for transgenic mouse strains relevant to musculoskeletal studies. Overall, the Mouse Genetic Models Core will maximize the benefit of existing mouse genetic resources through subsidized services to expedite musculoskeletal studies at Washington University.