Skin Cell Culture Core

作为DAB皮肤病研究中心（SDRC）的一部分，DAB SDRC皮肤细胞培养的使命 核心（SCCC）是提供有价值的资源，服务和研究技术的培训，以促进 皮肤生物学的跨学科研究。在过去的4年里，我们的核心服务已成为一个至关重要的 资源的多元化社区的35 UAB SDRC成员和12名新的调查员代表22 依靠我们的专业知识来促进和支持与皮肤生物学相关的研究。 SCCC的教师和研究人员已经建立了在生成和维护的专业知识， 纯化的表皮和真皮原代细胞亚群，主要来源于鼠和人皮肤组织，或 活检样品，在单层或器官型培养物中。我们建议继续支持和促进 UAB的皮肤研究具有以下具体目标： 1)为中心提供来自小鼠和人组织的表皮和真皮细胞类型的中心来源 以成本效益的方式。 2)保持质量控制措施，确保分发标准化的无污染 cultures. 3)提供专业知识，在产生专门的细胞培养系统和分离皮肤细胞， 人类或老鼠来源。 4)优化小鼠骨髓瘤细胞的长期连续培养和永生化方法 来源于野生型和突变型小鼠品系的角质形成细胞，以适应 SDRC成员。 通过以鼓励新探索和创新的成本提供各种细胞类型，SDRC SCCC 对于提高质量和扩大皮肤研究的广度至关重要， 在过去的四年里，有多名UAB研究人员。我们建议继续提供这项重要的服务，并加以改善。

As part of the DAB Skin Diseases Research Center (SDRC), the mission of the DAB SDRC Skin Cell Culture Core (SCCC) is to provide valuable resources, services, and training in research techniques for promoting interdisciplinary research in cutaneous biology. Over the last 4 years, our core services have become a vital resource to a diverse community of 35 UAB SDRC members and 12 new investigators representing 22 departments who depend upon our expertise to facilitate and support studies relating to cutaneous biology. The SCCC faculty and research staff have established expertise in the generation and maintenance of purified epidermal and dermal primary cell subsets, derived primarily from murine and human skin tissues or biopsy samples, in monolayer or organotypic cultures. We propose to continue to support and promote cutaneous research at UAB with the following specific aims: 1) To provide a central source of epidermal and dermal cell types from murine and human tissues to Center members in a cost efficient manner. 2) To maintain quality control measures that ensure the distribution of standardized contamination-free cultures. 3) To provide expertise in generating specialized cell culture systems and transfecting skin cells from either human or mouse sources. 4) To optimize methods for continuous long-term culture and immortalization protocols of murine keratinocytes derived from wild type and mutant mouse strains to accommodate increased demand by SDRC members. By providing various cell types at a cost that encourages new exploration and innovation, the SDRC SCCC has been essential for enhancing the quality and expanding the breadth of cutaneous research performed by multiple UAB investigators during last 4 years. We propose to continue and improve upon this vital service.