ANGIOGENIC ACTION OF NATURAL PRODUCTS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 关于裁决的通知出现了延迟。我们能够开始 雇用人员从事项目工作，并在项目结束后订购物资 开户日期为2010年10月。我的具体目标没有改变 由颁奖组件提供，旨在检查 阿霉素对内皮细胞增殖和迁移的影响，以及 低氧条件下血管内皮细胞生长因子的产生 和常氧内皮细胞；2)血管网络的形成 3)体内血管生成 更多的红心。我们已经使用了原代人体心脏微血管 内皮细胞执行特定目标1中描述的实验 (最初的具体目标是我们将使用原代小鼠细胞)。 使用原代人类细胞是有利的，因为它将增加 获得的结果具有临床意义，并减少了 将在这个项目中使用的动物。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. There was a delay in notification of the award. We were able to start hiring personnel to work on the project and ordering supplies after accounts opened in October 2010. My Specific Aims were not modified by the awarding component and are designed to examine effects of doxorubicin on 1) proliferation and migration of endothelial cells, and production of vascular endothelial growth factor (VEGF) by hypoxic and normoxic endothelail cells; 2) vascular network formation using in vitro angiogenesis assay; and 3) in vivo angiogenesis in an in vivo mose hearts. We have used primary human cardiac microvascular endothelial cells to perform experiments delineated in Specific Aim 1 (original Specific Aim stated we would be using primary mouse cells). Using primary human cells is advantageous because it will increase clincial significance of obtained results, and reduce the number of animals that will be used in this project.