INBRE-2 SECRETED PROTEINS ENCODED IN THE FRANCISELLA PATHOGENICITY ISLAND

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 在拟议的研究中，我将调查的假设，弗朗西斯菌致病性岛编码的蛋白质，在感染过程中分泌和影响感染过程中分泌的效应蛋白。 弗朗西斯菌是一种兼性细胞内病原体，被列为生物恐怖主义的潜在代理人，其基因组中携带一个区域，该区域对细胞内生长和毒力至关重要。在弗朗西斯菌致病岛（FPI）的这个区域中编码的一些蛋白质与六型分泌系统（TSSS）的部分具有同源性。我推测，FPI不仅编码分泌机制，但也分泌效应蛋白的TSSS。我将通过筛选潜在的分泌蛋白来验证这一假设，并描述这些因子在宿主细胞中的时空调控。此外，我将测试这些因素是否参与所描述的弗朗西斯菌的免疫调节能力。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In the proposed studies I will investigate the hypothesis that the Francisella Pathogenicity Island encodes proteins that are secreted during infection and influence the infection process as secreted effector proteins. Francisella, a facultative intracellular pathogen that is listed as a potential agent of bioterrorism, carries a region in its genome that is crucial for intracellular growth and virulence. Some proteins encoded in this region of the Francisella Pathogenicity Island (FPI) have homologies to parts of a Type Six Secretion System (TSSS). I hypothesize that the FPI does not only encode secretion machinery but also secreted effector proteins of this TSSS. I will test this hypothesis by screening for potentially secreted proteins and describe the temporal and spatial regulation of these factors in the host cell. In addition I will test if these factors are involved in the described immune regulatory ability of Francisella.