Paramagnetic Microbead-based Surface Plasmon Microarray Detection of Toxins and T

描述（由申请人提供）：拟议的工作将产生一个特征原型仪器，该仪器将有助于在少量样品制备的情况下，在少量人体血液、尿液、唾液和其他容易获得的生物液体中识别广泛的分析物。该仪器将结合两种相关的分析方法，光栅耦合表面等离子体共振分析和表面等离子体增强荧光，以提供宽的动态范围和高灵敏度的测量。分析物的选择性是通过使用捕获配体如抗体、适体等来实现的。所建议的原型所需的样本量约为0.3至0.5 ml。所建议的仪器将被设计为同时执行多个（约1000）分析。包金的超顺磁性纳米球将通过在受控磁场中传输分析物到捕获点来增加测量的选择性。该仪器将通过使用两种常见的毒物和一种细菌毒素来表征。软件控制的自动化将使仪器易于使用，适合在实验室外使用。该仪器将提供一种廉价而有效的方法来直接识别或监测可疑的毒物。

DESCRIPTION (provided by applicant): The proposed work will result in a characterized prototype instrument that will facilitate the identification of a broad range of analytes in small volumes of human blood, urine, saliva and other readily obtainable biological fluids with little sample preparation. The proposed instrument will incorporate two related analytical approaches, grating-coupled surface Plasmon resonance analysis and surface plasmon-enhanced fluorescence to provide a broad dynamic range coupled with highly sensitive measurements. Analyte selectivity is achieved through the use of capture ligands e.g. antibodies, aptamers, etc. Sample volumes required for the proposed prototype are on the order of 0.3 to 0.5 ml. The proposed instrument will be designed to perform multiple (approx.1000) analyses simultaneously. Gold-clad superparamagnetic nanobeads will be used to increase the selectivity of measurements by transporting analyte to capture sites in a controlled magnetic field. The instrument will be characterized through the use of two commonly encountered toxicants and a bacterial toxin. Software-controlled automation will make the instrument easy to use and suitable for use out of the laboratory. The instrument will provide an inexpensive and efficient way to directly identify or monitor suspected toxicants. PUBLIC HEALTH RELEVANCE: There is a critical need for highly sensitive and specific techniques capable of defining the exposure levels of human and animal populations to the broad range of toxins and toxicants found in the environment. We propose to develop an instrument that will meet this need without many of the limitations that constrain existing technologies. The proposed approach will perform large number (up to a thousand) simultaneous analyses in less than hour. The analyses will identify and quantitate the presence or absence of hundreds of potential toxicants that a patient might encounter without foreknowledge of the contaminant's identity. The technology upon which the proposed instrumentation is based is suitable for use in a clinical laboratory and, with further development extendable to a point-of-care instrument. The instrument will require small sample volumes of biological fluids such as blood, urine or saliva.