Leica SP5 confocal microscope for live-cell imaging

描述（由申请人提供）：本申请的目的是为宾夕法尼亚州立好时医学院核心成像设施购买一台新的共聚焦显微镜。虽然我们的核心设施可供医学院的所有研究人员使用，但提交此请求是为了满足一组nih资助的主要用户的特定需求。这台新显微镜可以进行我们现有设备无法完成的三种类型的研究。首先，因为显微镜将被专门配置为活细胞研究，我们可以满足快速增长的需求，更复杂的设备专用于这种实验方法。该配置将能够精确执行时移研究，荧光共振能量传输（FRET）和光漂白后荧光恢复（FRAP）。其次，显微镜还将有一个串联扫描仪，以提供高分辨率和高速扫描选项。这些特征将是至关重要的高分辨率成像实验，将允许离子流动和线粒体状态的动态研究。第三，该仪器将允许同时激发和检测多达五种不同的荧光团。这种新型显微镜的可用性将提高执行密集操作的能力

DESCRIPTION (provided by applicant): The objective of this application is to purchase a new confocal microscope for the Penn State Hershey College of Medicine Core Imaging Facility. Though our core facilities are available to all investigators in the College of Medicine, this request is submitted to meet the specific needs of a group of NIH-funded major users. This new microscope will permit three types of study that cannot be accomplished with our current facility. First, because the microscope will be configured specifically for live-cell studies, we can meet the rapidly growing need for more sophisticated equipment dedicated to this experimental approach. The configuration will enable precise execution of time lapse studies, fluorescence resonance energy transmission (FRET) and fluorescent recovery after photobleaching (FRAP). Second, the microscope will also have a tandem scanner to provide both high resolution and high speed scanning options. These features will be vital for high resolution imaging experiments and will allow dynamic studies of ion flow and mitochondrial status. Third, the instrument will allow the simultaneous excitation and detection of up to five different fluorophores. The availability of this new microscope will improve the ability to perform intensive live-cell imaging projects focusing on a variety of health issues including viral infections, cance, diabetes and its complications (primarily diabetic retinopathy and muscle protein synthesis), heart disease, degenerative diseases of the central and peripheral nervous system (including Parkinson's disease, Alzheimer's disease, restless leg syndrome and eye diseases such as glaucoma). The projects will use the equipment to image rapid events in live cells or explant tissue transfected with fluorescent protein markers, or labeled with metabolic dyes, to determine changes in protein expression, trafficking and redistribution in response to pharmacological, environmental or genetic manipulations. PUBLIC HEALTH RELEVANCE: The confocal microscope requested will have broad relevance to human health because it will enable us to study rapid interactions between molecules in living cells, in real time and thus aid determination of their function in health and disease. This instrument will enhance a wide variety of public health projects being conducted at the Milton S. Hershey Medical Center, including those related to cancer, diabetes, heart disease, viral infection and neurodegenerative diseases.