AB SCIEX 5500 QTRAP Mass Spectrometer for Lipidomics and Metabolomics Research

描述(由申请者提供)：弗吉尼亚联邦大学(VCU)拥有一批非常活跃且不断增长的资助研究人员，他们正在研究生物活性脂类的动态变化如何影响新陈代谢，以及调节正常和病理人类细胞功能的信号通路。请拨资金用于购置应用生物系统/SCIEX 5500四极线性离子陷阱质谱仪(5500 QTRAP)，这将加强脂肪组学/新陈代谢组学核心设施的分析能力。这一新的质谱仪系统将极大地提高我们从复杂样本中获取更多信息的能力，增加对低丰度、生物活性脂肪的定性和定量分析，大幅减少对患者样本的重要样本大小要求，并最终使研究人员能够研究亚细胞细胞器中的局部脂质代谢和功能。如本申请中所述，7名NIH资助的科学家和临床医生、拥有超过700万美元的NIH资助研究项目的主要用户以及来自8个不同部门的10名次要用户对该设备有特定需求。5500 QTRAP系统和现有的QTRAP系统将作为一个共享的研究资源运行，为我们正在进行的研究计划提供必要的支持，并促进新的协作研究计划，以识别、定量、鉴定和鉴定脂质，特别强调细胞信号和功能脂质组学。提高灵敏度、分析更复杂的脂类和代谢物、较小的样本量以及更多研究项目的日益增长的需求为这一要求提供了主要理由。该仪器将在杰里米·阿勒古德博士的技术指导下作为VCU的核心资源进行管理，他在脂质分析和核心资源管理方面拥有丰富的技术经验。Charles Chalfant博士(PI)和Sarah Spiegel博士将担任联席科学主任，并将与其他部门的主要用户一起在内部咨询委员会任职，以监督仪器的公平使用，造福于NIH资助的研究人员和大学研究环境。购买这种设备对于继续开发和实现脂质组学和代谢组学研究的全部潜力，以更好地了解VCU的正常细胞功能和人类疾病至关重要。 公共卫生相关性：弗吉尼亚联邦大学(VCU)拥有一批非常活跃且不断增长的资助研究人员，他们正在研究生物活性脂类的动态变化如何影响新陈代谢，以及调节正常和病理人类细胞功能的信号通路。需要资金购买新的质谱仪系统(例如，具有电喷雾和纳米离子源的应用生物系统/SCIEX 5500四极线性离子陷阱质谱仪)，以极大地增强VCU的脂质组学/代谢组学核心设施的分析能力，以帮助NIH资助的研究人员开展治疗人类疾病的科学研究。

DESCRIPTION (provided by applicant): Virginia Commonwealth University (VCU) has a very active and growing critical mass of funded investigators examining how dynamic changes in bioactive lipids influence metabolism and the signaling pathways that regulate normal and pathological human cellular functions. Funds are requested for the acquisition of the Applied Biosystems/SCIEX 5500 Quadrupole Linear Ion Trap Mass Spectrometer with electrospray and nanospray ion sources (5500 QTRAP) that will enhance the analysis capabilities of the Lipidomics/Metabolomics core facility. This new mass spectrometer system will dramatically increase the our ability to obtain more information from complex samples, increase qualitative and quantitative analysis of low abundance, bioactive lipids, drastically reduce sample size requirements important for patient samples, and finally, will allow researchers to investigate localized lipid metabolism and function in subcellular organelles. As described in this application, 7 NIH-funded scientists and clinicians, major users with over $7 M NIH supported research programs, and 10 minor users from 8 different departments have specific needs for this equipment. The 5500 QTRAP system together with the existing QTRAPs will be operated as a shared research resource to provide essential support for our ongoing research programs and to facilitate new collaborative research initiatives for lipid identification, quantitation, an characterization, with particular emphasis on cell signaling and functional lipidomics. These needs for enhanced sensitivity, analysis of more complex lipids and metabolites, smaller sample sizes, and increasing demand from more research projects provide the principal justification for this request. The instrument will be administered as a VCU core resource under the technical direction of Dr. Jeremy Allegood, who has extensive technical experience in lipid analysis and administration of core resources. Drs. Charles Chalfant (the PI) and Sarah Spiegel will serve as co- Scientific Directors and will serve on the Internal Advisory Committee together with major users from other departments to oversee equitable use of the instrument for the benefit of NIH-funded investigators and the university research environment. The purchase of this equipment is essential to continuing development and realization of the full potential of lipidomics and metabolomics research toward better understanding of normal cellular function and human disease at VCU. PUBLIC HEALTH RELEVANCE: Virginia Commonwealth University (VCU) has a very active and growing critical mass of funded investigators examining how dynamic changes in bioactive lipids influence metabolism and the signaling pathways that regulate normal and pathological human cellular functions. Funds are requested for the acquisition of a new mass spectrometer system (e.g. Applied Biosystems/SCIEX 5500 Quadrupole Linear Ion Trap Mass Spectrometer with electrospray and nanospray ion sources) to dramatically enhance the analysis capabilities of the Lipidomics/Metabolomics core facility at VCU to aid NIH-funded investigators undertake scientific research toward curing human diseases.