Clinical Specimen, Data Collection, and Expression Array Core

本计划项目中的四个项目都有一个共同的主题，即开发细胞免疫的新方法。 和基因治疗，并使用斑马鱼模型，以加快改善理解和治疗 肌肉萎缩症Pi程序有着悠久而广泛的互动历史， 多年来交换了许多想法、活检样本和其他试剂。该项目进一步 加强这些合作，以产生超出任何一个范围的协同成果。 实验室临床样本、数据采集和表达阵列核心（核心B）的服务包括 这是项目实现这些目标的重要手段。核心B允许增加 效率，并为每个项目和整个计划的数据分析提供标准化。 为此，核心B的目标旨在最大限度地提高效率，并最大限度地减少行政管理和 以及技术努力和费用。目标1。开展招募参与者所需的所有活动， 计划项目，包括以下内容：a）确定并登记计划项目的参与者; B） 从参与者那里获得全面的数据和标本，包括临床信息，血液样本， 和肌肉组织样本; c）获取对照组织和标本; d）对临床和 （e）维护人类发展国家方案网站（服务项目：1、2、3、4，核心C）。目的 2.为项目进行基因表达阵列分析：a）准备斑马鱼胚胎， 全鱼、细胞和肌肉样品用于基因表达分析，分离mRNA，并合成标记的 用于杂交至Affyphase GeneChip阵列和/或Illumina Sentrix BeadChip的cDNA/cRNA; B） 将cDNA杂交到Affyssin GeneChip阵列和/或Illumina Sentrix BeadChip;和 基因表达数据到ProjectManager，一个HNDP数据库，用于在Core C中进行分析。（服务项目：1、2、3、 核心C）。核心B将与核心C广泛地相互作用，在核心C中，对基因表达的大部分分析都是在核心C中进行的。 数据将发生。

The four projects in this Program Project share the common theme of developing newer approaches to cell and gene therapy, and using zebrafish models to accelerate improvements in understanding and treating the muscular dystrophies. The Program Pi'shave a long and extensive history of interaction and have exchanged many ideas, biopsy samples, and other reagents over many years. This Program Project further strengthens these collaborations to produce synergistic results that are beyond the scope of any one laboratory. The services of the Clinical Specimen, Data Collection, and Expression Array Core (Core B) are an important means by which the Program Project will meet these goals. Core B allows for increased efficiency and provides standardization to the analysis of data for each Project and the Program overall. Toward this end, the Aims of Core B are designed to maximize efficiency and minimize both administrative and technical effort and expense. Aim 1. To carry out all activities necessary for participant recruitment into the Program Projects, including the following: a) identify and enroll participants for the Program Project; b) acquire comprehensive data and specimens from participants, including clinical information, blood samples, and muscle tissue samples; c) acquire control tissues and specimens; d) catalogue and track the clinical and diagnostic data in a database; and e) maintain the HNDP web site (Projects served: 1, 2, 3, 4, Core C). Aim 2. To perform gene expression array analysis for the Program Project: a) Prepare zebrafish embryos and whole fish, cells, and muscle samples for gene expression analysis, isolate mRNA, and synthesize labeled cDNA/cRNA for hybridization to the Affymetrix GeneChip array and/or the Illumina Sentrix BeadChip; b) Hybridize cDNA to the Affymetrix GeneChip array and/or the Illumina Sentrix BeadChip; and c) Transfer gene expression data to ProjectManager, a HNDP database, for analysis in Core C. (Projects served: 1, 2, 3, Core C). Core B will interact extensively with Core C, where much of the analysis of the gene expression data will take place.