TAS::75 0850::TAS RAPID SCREENING OF INDIVIDUAL CELLS USING SINGLE CELL MASS SPE

将制造和测试使用基于纳米颗粒的MALDI矩阵实现的单细胞飞行时间质谱仪(SCMS)。与传统的基于表面受体的细胞表型和鉴定技术不同，SCMS系统将能够区分使用传统的基于抗体的方法无法区分的细胞类型。这种无标记的方法将提供单个细胞的分子信息，这将导致对异质细胞混合物中分子差异的新理解。另一种操作模式允许使用荧光、纳米颗粒或元素标签(例如，镧系元素)，以提供使用单细胞质谱仪作为检测器的高度多路复用的标记方法。SCMS还将具有连接到细胞仪的能力，该仪器可用于在进行质谱分析之前分离感兴趣的细胞群。在这里，用荧光抗体标记的细胞群体可以被进一步分析，以测量差异并识别标记细胞内的亚群体。

A Single Cell time-of-flight Mass Spectrometer (SCMS) that is enabled by the use of a nanoparticle based MALDI matrix will be fabricated and tested. Unlike traditional surface-receptor-based cell phenotyping and identification techniques, the SCMS system will be able to differentiate between cell types that are indistinguishable using traditional antibody based methods. The label-free method will provide molecular information on individual cells that will lead to a new understanding of the molecular variance in heterogeneous cell mixtures. Another mode of operation allows for the use of either fluorescent, nanoparticle or elemental tags (e.g. lanthanides), to provide a highly multi-plexed labeling method using the single cell mass spectrometer as the detector. The SCMS will also have the capability to be coupled to a cytometer which can be used to isolate cell populations of interest prior to mass spectrometry analysis. Here, cell populations labeled with a fluorescent antibody can be further analyzed to measure the variance and identify sub-populations within the tagged cells.