SBIR TOPIC 294 PHASE I - DEVELOPMENT OF GLYCOSYLATION-SPECIFIC RESEARCH REAGENTS

本工作说明书涉及拟议生产特定O&8208；GlcNAc抗体站点的合同。 响应小企业创新研究(SBIR)计划PHS2011；1.该项目寻求生产单克隆体 针对三个选定的癌症相关蛋白质靶标的抗体(单抗)，更具体地说，单抗将允许 通过O&#8208；GlcNAc部分检测这些蛋白质靶点内特定糖基化的存在。对于每个 在三(3)种选定的蛋白质中，将合成一种人工三方免疫原，动物将被免疫以 这种合成抗原的结合物和大量杂交瘤培养物将被生产出来进行筛选 特定的交互作用。将使用越来越严格的免疫分析制度来定义特异性和 单抗对产生试剂的反应性，该试剂将允许检测特定蛋白质的修饰 由O&#8208；GlcNAc；提供特定试剂，以允许检测特定的GlcNAc修饰 这些特定蛋白质中的位点将决定三方免疫原方法的效用，以及供应试剂 这将有助于阐明O&8208；GlcNAc修饰在细胞控制电路中的作用，包括那些可能 让这些特定的蛋白质参与肿瘤的发生。

This statement of work concerns a contract for the proposed effort ¿Production of Site‐Specific O‐GlcNAc Antibodies¿ in response to Small Business Innovation Research (SBIR) Program PHS2011‐1. The project seeks to produce monoclonal antibodies (MAbs) to three selected cancer‐related protein targets, and more specifically, MAbs that will that will permit detection of the presence of the specific glycosylation within these protein target sites by the O‐GlcNAc moiety. For each of the three (3) selected proteins, a synthetic ¿tripartite¿ immunogen will be synthesized, animals will be immunized to conjugates of this synthetic antigen, and a large number of hybridoma cultures will be produced to be screened for specific interactions. An increasingly stringent regime of immunoassays will be employed to define the specificity and reactivity of the MAbs towards producing reagents that will permit detection of the modification of the particular protein sites by O‐GlcNAc. The provision of specific reagents that allow detection of the O‐GlcNAc modification of the specific sites in these specific proteins will define the utility of the ¿tripartite¿ immunogen approach, as well as supply reagents that will aid in elucidating the role of O‐GlcNAc modification in cellular control circuits, including that those that may involve these specific proteins in oncogenesis.