Flow Cytometry Laboratory (Scientific Core)

虽然流式细胞术实验室是位于巴尔的摩的NIA内部研究计划内的实验室的科学使命的核心，但几个调查人员、实验室小组或项目占据了2009财年资源的重要部分。免疫学实验室(Li；Taub，Weng，Sen)的使用从60%-70%的分类能力到50%-70%的非分类(分析)能力，重点放在免疫状态、免疫调节、免疫抑制及其与发育和衰老的关系的人和动物(鼠)模型上。其他得到资助的项目包括心血管科学实验室(LCS：Boheler，ES细胞与心肌细胞分化；肖，报告基因表达)，遗传学实验室(LG：KO，ES发育模式)，神经科学实验室(LNS：Mattson，神经干细胞与神经发生中的报告基因表达)，临床调查实验室(Bernier)，分子老年学实验室(LMG：Gearhart，抗体多样性和体细胞超突变)，以及细胞和分子生物学实验室(LCMB；多参数细胞周期分析)。虽然实验室并不要求我们为其生成数据的所有出版物都是共同作者，但除了下面的参考书目外，以下清单还记录了工作组的出版物，其中包括本报告所述期间我们的核心人员所作的贡献。 阮H，翁NP。(2010)在整个成年阶段，IL-21优先促进IL-15介导的人CD28+CD8记忆T细胞的动态平衡增殖。J Leukoc Biol 87：43-9 Aggarwal M，Sommers JA，Shoemaker RH，Brosh RM Jr.(2011年)小分子抑制解旋酶活性损害沃纳综合征解旋酶在细胞对dna损伤或复制应激反应中的功能.学报，学报，U S，1081525-30. Aggarwal M，Sommers JA，Morris C，Brosh RM Jr.(2010)用EXO1描述了WRN解旋酶在复制应激反应中的功能。DNA修复9：765-76。 于琦，夏尔玛·A，戈什·A，森·JM。(2011)T细胞因子-1负向调节IL-17家族细胞因子的表达，保护小鼠免受实验性自身免疫性脑脊髓炎的侵袭。j免疫。186：3946-52。 J，Sanghvi M，O‘Connell M，Krzysik-Walker S，Moaddel R，Bernier M.(2011年)。大麻素受体反向激动剂AM251通过使雌激素相关受体(ERR)蛋白失稳来调节EGF受体及其配体的表达。BrJ Parmacol。3月30日。DOI：10.1111/j.1476-5381.2011.01384.x.EPub领先于印刷品 Maul RW，Saribasak H，Martomo SA，McClure RL，Yang W，Vaisman A，Gramlich HS，Schatz DG，Woodgate R，Wilson DM Third，Gearhart PJ。2011年，尿嘧啶残留量依赖于免疫球蛋白基因可变区和转换区中的脱氨酶AID。12(1)：70-6。

While the Flow Cytometry Laboratory functions as a core for the scientific mission of the laboratories located within the NIA Intramural Research Program in Baltimore, several investigators, laboratory groups, or projects accounts for a significant portion of FY2009 resources. Usage by the Laboratory of Immunology (LI; Taub, Weng, Sen) varies between 60-70% of sorting and 50-70% of non-sorting (analytical) capacities, focusing on human and animal (mouse) models of immune status, immunoregulation, immunosuppression, and their relation to development and aging. Other supported projects involve those from the Laboratory of Cardiovascular Sciences (LCS: Boheler, ES cells and cardiomyocte differentiation; Xiao, reporter gene expression), Laboratory of Genetics (LG: Ko, patterns of ES development), Laboratory of Neuroscience (LNS: Mattson, reporter gene expression in neural stem cells and neurogenesis), Laboratory of Clinical Investigation (Bernier), Laboratory of Molecular Gerontology (LMG: Gearhart, antibody diversity and somatic hypermuations), and Laboratory of Cellular and Molecular Biology (LCMB; multiparameter cell cycle analysis). While the lab does not require co authorship on all publications for which we generate data, the following list, in addition to the bibliography below, documents publications from the IRP that include contributions from our core for this reporting period. Nguyen H, Weng NP. (2010) IL-21 preferentially enhances IL-15-mediated homeostatic proliferation of human CD28+ CD8 memory T cells throughout the adult age span. J Leukoc Biol 87:43-9. Aggarwal M, Sommers JA, Shoemaker RH, Brosh RM Jr. (2011) Inhibition of helicase activity by a small molecule impairs Werner syndrome helicase (WRN) function in the cellular response to DNA damage or replication stress.Proc Natl Acad Sci U S A. 1081525-30. Aggarwal M, Sommers JA, Morris C, Brosh RM Jr. (2010) Delineation of WRN helicase function with EXO1 in the replicational stress response. DNA Repair 9:765-76. Yu Q, Sharma A, Ghosh A, Sen JM. (2011) T cell factor-1 negatively regulates expression of IL-17 family of cytokines and protects mice from experimental autoimmune encephalomyelitis. J Immunol. 186:3946-52. Fiori J, Sanghvi M, O'Connell M, Krzysik-Walker S, Moaddel R, Bernier M. (2011). The cannabinoid receptor inverse agonist AM251 regulates expression of EGF receptor and its ligands via destabilization of estrogen-related receptor (ERR) α protein. Br J Parmacol. Mar 30. doi: 10.1111/j.1476-5381.2011.01384.x. Epub ahead of print Maul RW, Saribasak H, Martomo SA, McClure RL, Yang W, Vaisman A, Gramlich HS, Schatz DG, Woodgate R, Wilson DM 3rd, Gearhart PJ. (2011) Uracil residues dependent on the deaminase AID in immunoglobulin gene variable and switch regions.Nat Immunol. 12(1):70-6.