ELECTRON PARAMAGNETIC RESONANCE (EPR) SPECTROSCOPY FACILITY

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 EPR(也称为电子自旋共振)谱是基于在外部电磁场的存在下，自由基或顺磁物种吸收微波能量，导致电子自旋态的跃迁。齐默尔曼博士在UNMC细胞和综合生理学系的实验室配备了一台Bruker E-Scan台式EPR光谱仪(见图)。与EPR可检测的、对自由基敏感的环羟胺(通常被称为“自旋探针”)相结合，E-SCAN可以并且已经被用于测量各种生物样本中的自由基(例如，超氧化物、羟基自由基)的水平，包括培养细胞、培养基、血液和隔离组织。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. EPR (also referred to as Electron Spin Resonance) spectroscopy is based on absorption of microwave energy by free radicals or paramagnetic species in the presence of an external electromagnetic field resulting in the transition of electron spin states. Dr. Zimmerman's laboratory in the Department of Cellular and Integrative Physiology at UNMC houses a Bruker E-Scan Table Top EPR spectrometer (see figure). In combination with EPR-detectable, radical-sensitive cyclic hydroxylamines, often referred to as "spin probes", the E-Scan can be and has been used to measure levels of free radicals (e.g. superoxide, hydroxyl radical) in various biological samples including cultured cells, culture media, blood, and isolated tissue.