SULFUR OXIDATION IN C ELEGANS BY OXIDATIVE STRESS AS COMPARED TO THE AGING PROC

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 线虫作为一种模式生物，由于其寿命短，交配容易控制，非常适合于衰老研究。雅各布斯实验室在线虫和衰老方面进行了广泛的研究，最近的工作是研究氧化应激的作用。他们和其他人一起开发了OxICAT系统，用于定位氧化还原活性硫醇蛋白质组。他们的结论表明，尽管氧化应激和衰老都会导致可逆氧化的半胱氨酸残基水平相当，但目标蛋白是不同的。然而，他们目前的方法只能将半胱氨酸氧化成二硫化物，而不能将半胱氨酸氧化成硫酸或磺酸。与雅各布斯实验室合作，我们建议使用硫XANES来绘制总硫氧化状态以及由于老化过程而发生的不可逆硫氧化量，并与氧化应激相比较。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. C. elegans as a model organism is well suited for studies in aging due to its short life span and easily controlled mating. The Jakobs lab has worked extensively on C. elegans and aging, most recently working on the role of oxidative stress. They, with other have developed the OxICAT system for mapping the redox active thiol proteome. Their conclusions show that while oxidative stress and aging both lead to comparable levels of reversibly oxidized cysteine residues, target proteins are different. However, their current methods can only map cysteines oxidized to disulphides and not cysteines oxidized to sulfunic or sulfonic acids. In conjunction with the Jakobs lab, we propose to use Sulfur XANES to map the total sulfur oxidation state and the amount of irreversible sulfur oxidation that happens as a result of the aging process compared to that of oxidative stress.