MASS SPECTROMETRY OF SIGNALLING LIPIDS

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 合作者希望对细胞信号脂质进行质谱分析。他们正在评估与IgE受体介导的信号传导相关的磷脂酰肌醇二磷酸（PIP 2）在质膜中的分布。他们已经获得了一些初步数据，并与McLafford教授一起发表了一篇论文，但这篇研究超出了他的常规研究范围，因此他将研究人员介绍给了BUSM资源。他们想使用的方法与一些已发表的工作相似，但这里提出的工作的难度更大。他们寻求的主要结果是将他们目前的生化数据与质谱中观察到的某些峰相关联。量化不同的PIP 2种类也应该是非常有趣的。在BUSM获得了一些初步数据，康奈尔大学的学生已经访问了参与进一步的分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The collaborators would like to carry out mass spectrometric of cell signaling lipids. They are evaluating phosphatidyl inositol bis phosphate (PIP2) distributions in plasma membranes related to IgE receptor mediated signaling. They have already obtained some preliminary data and have published a paper with Prof. McLafferty, but this are of research is outside of his usual research, so he has referred the investigators to the BUSM Resource. The approach theywould like to use parallels that of some published work, but the difficulty of the work proposed here is greater. The primary result they seek is correlating their present biochemical data to certain peaks observed in the mass spectra. It also should be very interesting to quantify different PIP2 species. Some initial data has been obtained at BUSM and the Cornell student has visited to participate in further analyses.