MODELING ORGANELLE TRAFFICKING

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 该项目的目的是了解微管(MT)依赖和肌动蛋白细丝(AF)转运之间的协调机制，使用鱼类黑素载体作为实验系统。这些大细胞的唯一功能是快速和同步地重新分布数以千计的膜结合的细胞器，即色素颗粒，它们聚集在细胞中心或均匀地重新分散在细胞质中。聚集过程中的色素重新分配涉及到MTS的负端的运输。色素弥散结合了最初向MT+末端的快速移动和沿AFS的缓慢扩散样移动，导致色素在细胞质中均匀分布。因此，色素的运输需要不同细胞骨架特异性和极性的多个马达活动的协调。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The goal of this project is to understand the mechanisms for the coordination between microtubule (MT)-dependent and actin filament (AF) transport using fish melanophores as an experimental system. The only function of these large cells is fast and synchronous redistribution of thousands of membrane-bounded organelles, pigment granules, which aggregate at the cell center or redisperse uniformly throughout the cytoplasm. Pigment redistribution during aggregation involves transport to the minus ends of MTs. Pigment dispersion combines initial rapid movement to the MT plus ends and slow diffision-like movement along the AFs, which results in the homogeneous distribution of pigment in the cytoplasm. Therefore pigment transport requires coordination of the activities of multiple motors of different cytoskeletal specificity and polarity.