EXAMINATION OF LIRKO ISLETS AND SERUM USING QUANTITATIVE PROTEOMIC APPROACHES

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 糖尿病研究（1型和2型）的中心目标是产生大量功能性胰岛或β细胞用于替代治疗。因此，促进β细胞再生的机制和因素的基本知识对于计划策略以保存和增强体内β细胞群或在体外产生用于移植的β细胞是必不可少的。 该项目应用定量蛋白质组学方法来确定新的血液循环因子和胰岛蛋白参与β细胞增殖在一个独特的小鼠模型，肝脏特异性胰岛素受体敲除（LIRKO）小鼠，表现出20 - 30倍的β细胞质量增加，以响应胰岛素抵抗。 该项目需要资源的高灵敏度、高分辨率LC-FTICR能力，以比较定量表征胰岛素受体肝特异性敲除小鼠（LIRKO）的胰岛蛋白和血清蛋白。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. A central goal of diabetes research (type 1 and type 2) is to generate large numbers of functional pancreatic islets or beta-cells for replacement therapy. Therefore, a fundamental knowledge of mechanisms and factors that promote beta-cell regeneration is essential for planning strategies to preserve and enhance beta-cell mass in vivo or to generate beta-cells in vitro for transplantation. This project applies quantitative proteomics approaches to identify new blood circulatory factors and islet proteins involved in beta-cell proliferation in a unique mouse model, the liver-specific insulin receptor knockout (LIRKO) mouse, which exhibit 20- to 30-fold increase in beta-cell mass in response to insulin resistance. This project requires high sensitivity, high resolution LC-FTICR capability from the resource for comparative quantitative characterization of the pancreatic islet proteins and serum proteins from mice with liver specific knockout of insulin receptor (LIRKO).