Autoimmunity Against Novel Antigens in Neuropsychiatric Dysfunction

描述（由申请人提供）：我们提出表征导致紧张症、自闭症行为和其他神经精神障碍的对神经元细胞表面和突触蛋白的自身免疫反应。2007年，我们报道了一组年轻女性，她们急性发展为精神病行为或精神分裂样症状，随后出现记忆缺陷、紧张症、运动异常和自主神经功能障碍。使用我们优化的一组技术，我们发现所有患者都有针对N-甲基-D-天冬氨酸受体（NMDAR）NR 1亚基的抗体，NMDAR是一种参与突触传递和可塑性的谷氨酸受体。在这些患者中，约60%的免疫反应的触发因素是卵巢畸胎瘤，其中含有表达NMDAR的异位神经组织。自该报道以来，患有这种疾病的患者数量迅速增加，并且应用于患有其他神经精神疾病的患者的类似临床和实验室策略已经导致发现了6种针对细胞表面/突触自身抗原的新型免疫应答，所述细胞表面/突触自身抗原包括AMPA受体（AMPAR）的GluR 1/2亚基、GABA（B1）受体（GABA（B1）R）、富含亮氨酸的胶质瘤失活蛋白1（LGI 1）和接触蛋白相关蛋白2（Caspr 2）。这项工作是重要的，因为：1）疾病影响年轻人，包括男性和儿童有或没有肿瘤; 2）他们对免疫治疗有反应; 3）一些抗体定义新的综合征; 4）抗原的表征导致明确的诊断测试;和5）患者抗体对靶受体/蛋白质的功能具有滴度依赖性和可逆性作用。总体而言，这些研究结果导致了一种假设，即许多病因不明的亚急性精神疾病，包括紧张症或自闭症行为，单独或与其他神经系统表现相关，可能是由影响细胞表面或突触部位神经递质受体的抗体介导的。我们将在三个目标中测试这个假设。(1)我们将选择患有以下3种疾病之一的患者，我们有针对细胞表面/突触蛋白的血清或CSF抗体的初步证据：具有紧张性特征的快速进行性神经精神疾病，儿童和成人获得性快速进行性自闭症行为谱，以及儿童和青少年边缘系统脑病。(2)我们将使用我们已经开发和优化的用于检测神经元细胞表面/突触抗原的高灵敏度方法来鉴定这3种疾病中的自身抗原;以及（3）我们将使用体外和体内研究来确定患者针对新型细胞表面/突触抗原的抗体如何影响神经元和突触的结构和功能，以及这些在抗体去除后如何恢复。我们将建立导致紧张症，自闭症特征和边缘系统脑病的自身免疫过程以及潜在的细胞和突触机制。识别自身免疫机制将导致改善儿童和成人这些疾病的治疗干预，这将对健康产生重大影响，并减少神经和神经精神疾病的负担。

DESCRIPTION (provided by applicant): We propose to characterize the autoimmune responses to neuronal cell surface and synaptic proteins that result in catatonia, autistic behaviors and other neuropsychiatric disturbances. In 2007, we reported a group of young women who acutely developed psychotic behavior or schizophrenia-like symptoms, subsequently followed by memory defects, catatonia, abnormal movements, and autonomic dysfunction. Using a set of techniques that we optimized, we found that all patients had antibodies against the NR1 subunit of the N- methyl-D-aspartate receptor (NMDAR), a glutamate receptor involved in synaptic transmission and plasticity. In about 60% of these patients, the trigger of the immune response was an ovarian teratoma that contained ectopic nervous tissue expressing NMDAR. Since that report, the number of patients with this disorder has rapidly increased, and similar clinical and laboratory strategies applied to patients with other neuropsychiatric disorders have resulted in the discovery of 6 novel immune responses to cell surface/synaptic autoantigens, including among others the GluR1/2 subunits of the AMPA receptor (AMPAR), the GABA(B1) receptor (GABA(B1)R), Leucine rich glioma inactivated 1 (LGI1) and Contactin associated protein 2 (Caspr2). This work is significant because: 1) the disorders affect young adults, including men and children with or without tumors; 2) they are responsive to immunotherapy; 3) some antibodies define new syndromes; 4) the characterization of the antigens has resulted in unambiguous diagnostic tests; and 5) patient antibodies have titer dependent and reversible effects on the function of the target receptors / proteins. Overall, these findings have led to the hypothesis that many subacute psychiatric disorders of unknown etiology, including catatonia or autistic behaviors, either alone or in association with other neurologic manifestations, are likely mediated by antibodies that affect neurotransmitter receptors at cell surface or synaptic sites. We will test this hypothesis in 3 goals. (1) We will select patients with one of 3 disorders for which we have preliminary evidence of serum or CSF antibodies to cell surface/synaptic proteins: rapidly progressive neuropsychiatric disorders with catatonic features, the spectrum of acquired rapidly progressive autistic behavior in children and adults, and limbic encephalopathy in children and adolescents. (2) We will identify the autoantigens in these 3 disorders, using highly sensitive methods we have developed and optimized to detect neuronal cell surface / synaptic antigens; and (3) We will use in vitro and in vivo studies to determine how patients' antibodies against novel cell surface/synaptic antigens affect neuron and synaptic structure and function, and how these recover after antibodies are removed. We will establish the autoimmune processes that lead to catatonia, autistic features, and limbic encephalopathy and the underlying cellular and synaptic mechanisms. Identification of autoimmune mechanisms will result in improved therapeutic interventions for these disorders in children and adults that will have a significant health impact and reduce the burden of neurological and neuropsychiatric disease.