XF96 Extracellular Flux Analyzer

描述：这项申请的目标是获得资金的海马XF96(细胞外通量，96孔)分析仪。最近，人们对细胞新陈代谢和能量利用的兴趣重新抬头，几乎影响了细胞生物学研究的所有领域。这包括代谢、神经退行性疾病和心血管疾病、癌症、药物安全性、基本细胞生理学、免疫学和炎症过程的研究。这一分析在不同学科中的重要性反映在为这项S10设备拨款申请而联合起来的研究人员的研究兴趣的范围中。这种新的兴趣在很大程度上是由各种“组学”分析推动的，这些分析经常识别动物模型和患者样本中细胞之间的能量使用差异。海马生物科学公司满足了这种新的兴趣，并产生了一系列机器，极大地提高了吞吐量和易用性。他们的仪器非常适合作为核心设施，因为用户可以迅速接受培训，分析和询问他们感兴趣的实验样品。XF96能够同时测量原代细胞或分离的线粒体上的糖酵解和氧化磷酸化。96孔微孔板格式允许较小的细胞样本尺寸，以在原位贴壁和悬浮细胞上实时测量多个分析物(目前是氧气和质子)。此外，最多四个测试化合物(药物或底物或激动剂或拮抗剂)可以自动添加到每个井中，并在添加每个试剂之前和之后进行测量。最后，这项技术通过在细胞生理学和疾病状态中打开新的洞察力和激活范例，填补了目前大多数研究实验室所缺乏的关键利基市场。

DESCRIPTION: The goal of this application is to obtain funding for a Seahorse XF96 (extracellular flux, 96 well) analyzer. A resurgence of interest in cell metabolism and energy use has recently impacted almost all areas of research on cell biology. This includes research into metabolic, neurodegenerative and cardiovascular diseases, cancer, drug safety, basic cell physiology, immunology and the processes of inflammation. The importance of this analysis among various disciplines is mirrored by the range in research interests of the investigators joining together for this S10 equipment grant application. Much of this renewed interest has been driven by the various "omic" analyses, which often identify variations in energy use among cells in both animal models and patient samples. Seahorse Bioscience has met this renewed interest and generated a series of machines that greatly improve the throughput and ease of use. Their instruments are perfect for a core facility as users can be rapidly trained to analyze and interrogate their experimental samples of interest. The XF96 is capable of measuring both glycolysis and oxidative phosphorylation simultaneously on primary cells or isolated mitochondria continuously in real time. The 96-well microplate format allows for smaller cell sample sizes to measure multiple analytes (currently oxygen and protons) in real time on adherent and suspension cells in situ. In addition, up to four test compounds (drugs or substrates or agonists or antagonists) may be added automatically to each well with measurements performed before and after addition of each reagent. Lastly, this technology fills a critical niche currently absent from most research labs by opening up new insights and paradigms of activation in cell physiology and disease states.