Ohio State Neuroscience Center Core

描述（由申请人提供）：俄亥俄州立大学（OSU）神经科学中心为旨在分析和治疗神经系统疾病的研究提供支持，重点是动物模型。该中心是俄勒冈州立大学和全国儿童医院43名神经科学家（25名NINDS资助）的合作成果。这些pi主要研究神经系统疾病的各个方面，主要关注四个领域：运动神经元疾病、神经肌肉疾病、CNS和PNS损伤以及脑肿瘤。这些pi横跨俄勒冈州立大学的许多部门、中心和研究所，支持基础和转化神经科学研究，使该中心成为校园神经科学活动的核心。中心的优先事项与俄勒冈州立大学的神经科学签名项目保持一致，并可以利用该项目，该项目耗资数百万美元，支持神经科学作为校园五大增长领域之一。此外，该中心与新授予俄勒冈州立大学医学中心的临床和转化科学奖保持一致。为支持神经系统疾病模型的开发、表征和分析，本提案支持5个研究核心。核心A（行政）将监督俄勒冈州立大学神经科学中心。核心B（遗传学）将支持转基因/基因敲除小鼠的发展和斑马鱼的使用，重点是传统和新的遗传方法。此外，该核心将支持一个基因组操作设施，专注于锌指核酸酶和BAC重组技术，以产生新的动物模型。核心C （CNS/PNS损伤和啮齿动物行为）将提供设备和技术专长来生成损伤模型，并分析与这些模型相关的行为缺陷。Core D（生理学）将为电生理学和肌肉生理学提供设备和技术援助，以分析这些模型。Core E（成像）将以更低的成本和技术援助支持共聚焦和核磁共振成像。Core F （Xenograft）将提供设备和技术援助，以生成脑肿瘤的小鼠模型。这些独特的核心将为pi提供任何单一实验室都无法获得的集中设备和技术专长，从而大大加强俄勒冈州立大学校园的神经科学研究。

DESCRIPTION (provided by applicant): The Ohio State University (OSU) Neuroscience Center provides support for studies aimed at analysis and treatment of neurological disorders with an emphasis on animal models. The Center is a collaborative effort of 43 neuroscientists (25 NINDS funded) from OSU and Nationwide Children's Hospital. The majority of these PIs work on aspects of nervous system disorders with four major focus areas being motoneuron disease, neuromuscular disease, CNS and PNS injury, and brain tumors. These PIs span numerous departments, centers, and institutes at OSU that support basic and translational neuroscience research making the Center a nucleus of neuroscience activity on campus. Center priorities align with and can leverage the Neuroscience Signature Program at OSU, a multi-million dollar effort supporting Neuroscience as one of five growth areas on campus. In addition, the Center aligns with the newly granted Clinical and Translational Science Award to the OSU Medical Center. To support the development, characterization and analysis of nervous system disease models, this proposal supports 5 research Cores. Core A (Administrative) will oversee the OSU Neuroscience Center. Core B (Genetics) will support the development of transgenic/knockout mice and the use of zebrafish with an emphasis on traditional and novel genetic approaches. In addition this Core will support a Genome Manipulation Facility focusing on zinc-finger nuclease and BAC recombineering techniques for the generation of new animal models. Core C (CNS/PNS Injury and Rodent Behavior) will provide the equipment and technical expertise to generate injury models and to analyze behavioral deficits associated with these models. Core D (Physiology) will provide equipment and technical assistance for both electrophysiology and muscle physiology to analyze these models. Core E (Imaging) will support both Confocal and MRI imaging with reduced costs and technical assistance. Core F (Xenograft) will provide equipment and technical assistance to generate mouse models of brain tumors. These unique Cores will provide PIs with access to centralized equipment and technical expertise unavailable in any single lab, thus substantially enhancing neuroscience research on the OSU campus.