TUSM/T-NEMC CENTER FOR NEUROSCIENCE RESEARCH - Imaging Core

理论基础和使命。先进显微镜技术的发展是背后的驱动力 生物研究和医学成像的进展。在过去的几十年里，精细化和商业化 共焦/多光子显微镜的发展以及荧光团的发展(例如 GFP衍生品、变色龙和许多其他)导致了生物显微镜的复兴(Denk et 等，科学248,1990；活塞，Trends Cell Biol 9,1999；Diaspro2002，Wiley-Liss；Van Roessel and Brand， NAT细胞生物学4,2002；吉普曼斯等人，科学312,2006)。然而，最新的成像方法通常需要 专业知识和仪器超出了大多数科学家个人的财力和技术能力。因此， 共享成像设施已成为大学研究核心的重要组成部分。 CNR成像和细胞分析核心是为了应对当代快速发展而开发的 生物显微镜和满足塔夫茨神经科学研究人员的成像优先事项。这个 CORE现在为塔夫茨神经科学家提供广泛的显微成像服务，包括广域 荧光显微镜和数字成像，共焦显微镜，双光子显微镜，活细胞成像， 电子显微镜和激光捕获显微解剖。除了标准 微观调查，调查人员利用核心设施进行动态 成像研究，如荧光共振能量转移(FRET)或 光漂白后荧光恢复(FRAP)实验。堆芯 设备和服务将在后面的章节中介绍。

Rationale and Mission. The development of advanced microscopy techniques is a driving force behind advances in biological research and medical imaging. In the past several decades, the refinement and commercialization of confocal/multiphoton microscopes along with advances in fluorophore development (such as GFP derivatives, Chameleons and many others) have led to a renaissance in biological microscopy (Denk et al., Science 248, 1990; Piston, Trends Cell Biol 9, 1999; Diaspro, 2002, Wiley-Liss; Van Roessel and Brand, Nat Cell Biol 4, 2002; Giepmans et al., Science 312, 2006). The newest imaging methods, however, often require expertise and instruments beyond the financial and technical capacity of most individual scientists. Thus, shared imaging facilities have become crucial components of university research cores. The CNR Imaging & Cell Analysis Core was developed in response to the rapid advances occurring in contemporary biological microscopy and to meet the imaging priorities of Tufts neuroscience investigators. The core now provides Tufts neuroscientists with a broad range of microscopic imaging services including widefield fluorescence microscopy & digital imaging, confocal microscopy, 2-photon microscopy, live-cell imaging, electron microscopy, and laser capture microdissection. In addition to standard microscopic investigations, investigators use core facilities to perform dynamic imaging studies such as fluorescence resonance energy transfer (FRET) or fluorescence recovery after photobleaching (FRAP) experiments. Core equipment and services are described in a later section.