Neurophysiologic

脑电活动、氧合状态、温度和血液灌注的神经生理学测量严格地反映了脑功能和健康，并为MR研究提供了重要的机制见解。适当利用这些神经生理学措施与MR结合需要大量的专业知识与适当的生理监测和动物控制。核心3的总体目标是支持将最先进的神经生理学测量与NINDS中的MR研究以及NIH支持的相关临床和基础神经科学研究相结合。在上一个周期中，我们通过开发和实施广泛的光学和电学测量方法来实现这一目标，包括细胞外记录（单个微电极，电极阵列），磷光淬灭组织氧合（pO 2），热电偶线温度监测和激光多普勒血流仪血流。核心3的合格用户组由NINDS资助的7个Pis组成，他们沿着与其他11个NIH资助的Pis一起使用多模态神经生理学和其他跨模态方法来解决基础和临床神经科学中的基本问题。核心3对耶鲁大学神经科学界的影响体现在它为15项NIH赠款和7项新的研究计划提供的支持，这些研究计划导致了赠款和论文，为5名年轻的神经科学家提供了先进的神经生理学方法的培训。（3个在NINDS PI实验室），以及在33篇论文中使用核心资源，导致355次引用（仅核心3就占所有核心106篇论文总引用的30%）。对于下一个周期，我们将继续为核心3用户实施，维护和支持创新的多模态神经生理学方法，支持新的研究计划，为核心3使用的神经科学Pis及其人员提供培训和指导，整合MR/神经生理学测量和项目特定数据分析的协同使用，实施新的核心3方法以支持神经科学Pis，并跟踪核心3活动，向NIH社区传播/共享资源。新实施的应用将包括利用多电极阵列和激光散斑方法来支持跨学科MR研究。

Neurophysiologic measures of brain electrical activity, oxygenation state, temperature, and blood perfusion critically reflect brain function and health and provide important mechanistic insight for MR studies. Appropriate utilization of these neurophysiologic measures in conjunction with MR requires substantial expertise with suitable physiological monitoring and control of animals. The overall goal of Core 3 is to support integration of state-of-the-art neurophysiological measurements with MR studies in NINDS and related NIH-supported clinical and basic neuroscience research. In the last cycle, we achieved this aim by developing and implementing a wide array of optical and electrical measurement methods including extracellular recordings (single microelectrodes, electrode arrays), tissue oxygenation (pO2) with phosphorescence quenching, temperature monitoring with thermocouple wires, and blood flow with laser Doppler flowmetry. The qualifying user group of Core 3 consists of 7 Pis funded by NINDS who along with 11 other NIH-funded Pis use multimodal neurophysiology and other cross-modal methods to address fundamental questions in basic and clinical neuroscience. The impact of Core 3 on the Yale neuroscience community is demonstrated by the support it provided to 15 NIH grants and 7 new research initiatives that led to grants and papers, the training in advanced neurophysiology methods provided to 5 young neuroscientists (3 in NINDS PI laboratories), and the use of Core resources in 33 papers that led to 355 citations (for Core 3 alone this represents 30% of the total citations for 106 papers for all Cores). For the next cycle, we will continue to implement, maintain, and support innovative multi-modal neurophysiology methods for Core 3 users, support new research initiatives, train and provide mentorship for neuroscience Pis and their personnel for Core 3 usage, integrate synergistic use of MR/neurophysiological measurements and project-specific data analysis, implement new Core 3 methods to support neuroscience Pis, and track Core 3 activities and disseminate/share resources to NIH community. The newly implemented applications will include utilizing multi-electrode arrays and laser speckle methods to support cross-disciplinary MR studies.