Exploring the role of microtubules in HIV-1 uncoating

描述（由申请人提供）：在人类免疫缺陷病毒（HIV）膜与靶细胞膜融合后，发生一系列事件以建立感染。具体来说，在生命周期的早期，病毒必须逆转录其RNA基因组，并诱导该基因组转运到核膜，以便随后的核输入和整合。在病毒基因组进入细胞核之前的某个时刻，病毒经历了一个鲜为人知的脱壳过程，定义为从病毒核糖核蛋白复合物中拆卸组装的衣壳结构。然而，脱涂层的确切机制仍然难以捉摸。这一应用的基础是一个假设，即成熟的、组装好的病毒衣壳晶格与微管马达蛋白和/或微管相关蛋白（MAPs）之间的相互作用影响了涂层的剥离。我们提供了MT破坏抑制HIV-1脱衣的证据，并建议利用检测脱衣、活细胞成像检测和衣壳结合检测来识别参与促进HIV-1脱衣的map。

DESCRIPTION (provided by applicant): Following human immunodeficiency virus (HIV) membrane fusion with the target cell membrane, a series of events occur to establish an infection. Specifically, early in the life cycle, the virus must reverse transcribe its RNA genome and induce the transport of this genome to the nuclear envelope for subsequent nuclear import and integration. At some point prior to the nuclear import of the viral genome, the virus undergoes the poorly understood process of uncoating, defined as the disassembly of the assembled capsid structure from the viral ribonucleoprotein complex. However, the precise mechanism of uncoating remains elusive. The foundation of this application is the hypothesis that uncoating is influenced by the interaction between the mature, assembled viral capsid lattice and microtubule motor proteins and/or microtubule- associated proteins (MAPs). We provide evidence that MT disruption inhibits HIV-1 uncoating and propose to utilize assays to measure uncoating, live cell imaging assays and capsid binding assays to identify the MAPs which are involved in facilitating HIV-1 uncoating.