Molecular Cytogenetic

项目总结（见说明）：分子细胞遗传学共享资源（MCSR）提供了用于制备适用于整个基因组的分子遗传学和细胞遗传学分析的人类和小鼠样本的工具。其目标是为癌症中心的研究人员提供在不同分辨率水平上分析基因组所需的全方位服务。这些服务包括建立EB病毒转化细胞系，从各种组织培养样品以及初级活检中分离DNA和mRNA。该设施与基因组学共享资源一起运作，为提供基因组材料进行分析创建直接管道。提供全套服务，从中期染色体的制备到定制设计的探针的生成以及用于检测拷贝数和结构染色体改变的杂交。MCSR提供从标准到光谱核型分析，从全染色体染色到基因组独特区域的基因座特异性探针的所有细胞遗传学分辨率水平的分析。MCSR人员接受培训，以设计针对研究者感兴趣区域的靶向基因座特异性探针。所有探头都是定制设计和内部生成的。MCSR还可以对石蜡包埋组织进行杂交，以及图像采集和完整的数据分析。

PROJECT SUMMARY (See instructions): The Molecular Cytogenetic Shared Resource (MCSR) provides tools for the preparation of human and murine samples suitable for molecular genetic and cytogenetic analysis of the entire genome. The goal is to provide Cancer Center investigators with the whole spectrum of services necessary to analyze the genome at various levels of resolution. These services include the establishment of EBV transformed cell lines and isolation of DNA and mRNA from a variety of tissue culture samples as well as primary biopsies. The facility operates in conjunction with the Genomics Shared Resource to create a direct pipeline for the provision of genomic material for analysis. A full panel of services is provided, from preparation of metaphase chromosomes to the generation of custom designed probes and hybridization for the detection of copy number and structural chromosomal alterations. The MCSR provides analyses at all cytogenetic levels of resolution from standard to spectral karyotyping and from whole chromosome paints to locus-specific probes for unique regions of the genome. MCSR personnel are trained to design targeted, locus-specific probes for regions of interest to investigators. All probes are custom designed and generated in-house. The MCSR also performs hybridizations on paraffin-embedded tissues as well as image acquisition and full data analysis.