Slip-flow chromatography

描述：该产品将是一种用于肽的nanoUHPLC的填充毛细管，可显著提高速度和效率。 目前用于高速和高效的商业柱使用小于2 mm的颗粒。拟议的技术使用0.2毫米以下的颗粒。 使用这种小颗粒用于UHPLC的能力是通过滑移流现象实现的，这在反相液相色谱中提供了增强的体积流速。 滑移流是一个突破，它克服了由移动的相中的抛物线速度分布引起的峰加宽的基本限制。 使用无孔亚0.2 mm颗粒还消除了颗粒内扩散，同时提供高的相比。 初步结果表明，亚微米无孔二氧化硅颗粒达到扩散极限的效率在蛋白质分离，和扩散极限预计将达到更快的扩散肽。 这给出了与长度无关的板数，允许长度小于2 cm的柱以高速给出100，000个板。 具体目标是1）优化粒径，2）优化分离长度，3）评估胰蛋白酶消化物的纳米LC-MS。

DESCRIPTION: The product will be a packed capillary for nanoUHPLC of peptides that provides a dramatic increase in speed and efficiency. Current commercial columns for high speed and efficiency use sub-2 mm particles. The proposed technology uses sub-0.2 mm particles. The ability to use such small particles for UHPLC is enabled by the phenomenon of slip flow, which gives enhanced volume flow rates in reversed-phase liquid chromatography. Slip flow is a breakthrough that overcomes the fundamental limit to peak broadening caused by the parabolic velocity profile in the mobile phase. Using nonporous sub-0.2 mm particles also eliminates intraparticle diffusion while giving high phase ratios. Preliminary results show that sub micrometer nonporous silica particles reach the diffusion limit for efficiency in protein separations, and the diffusion limit is expected to be reached for the faster diffusing peptides. This gives plate numbers that are independent of length, allowing columns less than 2 cm in length to give 100,000 plates with high speed. The specific aims are to 1) optimize the particle diameter, 2) optimize the separation length, and 3) evaluate the nano LC-MS of a tryptic digest.