Evaluation of fecal salmonella in symptomatic and asymptomatic dogs and cats

1人类感染沙门氏菌在美国是一个主要的健康问题， 2通过与狗和猫接触而接触这种毒剂的情况尚不清楚。而细菌学 对大型动物的粪便样本进行沙门氏菌培养是常规的，相似的 对小动物，特别是那些没有临床症状的小动物的诊断要少得多。 5经常做。沙门氏菌不能很好地与其他肠道细菌和常规 培养过程可能导致漏诊，特别是在污染严重的样本中。 已经对提高沙门氏菌敏感性的技术进行了大量研究。 8畜禽粪便和环境样品的回收。这些程序可以 在评估小动物粪便样本中是否存在这种试剂时，9被证明是有用的。 0个有症状和无症状的小动物患者在VMTH就诊 加州大学戴维斯分校将使用两种方法对粪便样本中是否存在沙门氏菌进行筛查 2浓缩程序。这些样本可以用聚合酶链式反应检测、传统培养或 3两者都是为了最大限度地提高沙门氏菌检测的灵敏度。确认的分离物将是 4个血清组和血清分型，以确定在这些人群中个别血清型的流行情况 5个种群。关于被测试动物的流行病学信息，可能提供对 6还将收集该制剂在狗和猫体内的生态情况。此信息将提供 7关于宠物种群中沙门氏菌基线水平的宝贵数据，特别是在 目标是评估流行率的变化，这可能与接触受污染的 9宠物产品或评估与宠物接触对人类的风险。

1 Salmonella infections in humans represent a major health concern in the U.S., and the risks of 2 exposure to this agent through contact with dogs and cats are unknown. While bacteriologic 3 cultures for Salmonella on fecal samples in large animals are performed routinely, similar 4 diagnostics in small animals, particularly those that have no clinical symptoms, are much less 5 frequently done. Salmonella does not compete well against other enteric bacteria and routine 6 culture procedures may result in missed diagnoses, particularly in heavily contaminated samples. 7 Much research has been conducted on techniques to enhance the sensitivity for Salmonella 8 recovery in livestock and poultry fecal and environmental samples. These procedures could 9 prove useful when assessing small animal feces samples for the presence of this agent. 0 Populations of symptomatic and asymptomatic small animal patients presented to the VMTH at 1 U.C. Davis will be screened for the presence of Salmonella in fecal samples using two 2 enrichment procedures. These samples can be tested using PCR testing, traditional culture, or 3 both in order to maximize sensitivity of Salmonella detection. Confirmed isolates will be 4 serogrouped and serotyped in order to characterize prevalence of individual serotypes in these 5 populations. Epidemiologic information on the animals tested that may provide insights into the 6 ecology of this agent in dogs and cats will also be collected. This information will provide 7 valuable data on the baseline level of Salmonella in the pet population, particularly when the 8 goal is to assess changes in prevalence that may be associated with exposure to a contaminated 9 pet product or to evaluate risks to humans from contact with their pets.