Magneto-Nano Diagnostic and Analytical Devices for Cancer

项目概述：研究项目2是围绕设计和翻译的研究， 应用新型可调磁性纳米粒子（MNP）、超灵敏磁性传感器芯片和高效磁性分选仪检测癌症生物标志物和细胞。如图N3.2.1所示，所得磁性纳米蛋白芯片平台将检测低至阿摩尔浓度的蛋白质生物标志物（目标1.A），并将用于监测肺癌的治疗反应（目标1.B）和卵巢癌的早期诊断（目标1.0）。所得到的磁筛（Aim 2.A）和可调MNP（Aim 2.B）将用于从肺癌患者或卵巢癌干细胞中收获循环肿瘤细胞，其捕获和释放效率前所未有地>90%（Aim 2.C），这对于癌症诊断和治疗都至关重要。这些罕见的细胞将通过单细胞蛋白质和核酸谱分析以及分泌组分析，使用目标1以及项目3（N3.3.5.2.1.3）中的纳米技术进行询问，以进一步阐明癌症途径和转移（目标3）。将纳米技术的这些进展应用于临床前模型和临床受试者将导致更好地理解和治疗癌症。

Project Overview: Research Project 2 is centered on the translational research of devising and applying novel and tunable magnetic nanoparticles (MNP), ultrasensitive magnetic sensor chips, and highly efficient magnetic sorters for cancer biomarkers and cells. As illustrated in Figure N3.2.1, the resulting magneto-nano protein chip platform will detect protein biomarkers down to attomolar concentrations (Aim 1.A) and will be used for monitoring therapeutic response of lung cancer (Aim 1.B) and early diagnosis of ovarian cancer (Aim 1.0). The resulting magnetic sifter (Aim 2.A) and tunable MNP (Aim 2.B) will be used to harvest circulating tumor cells from lung cancer patients or ovarian cancer stem cells with unprecedented capture and release efficiencies of >90% (Aim 2.C), critical for both cancer diagnosis and treatment. These rare cells will be interrogated with the nanotechnologies in Aim 1, as well as Project 3 (N3.3.5.2.1.3), by single cell protein and nucleic acid profiling and secretome analysis to further elucidate cancer pathways and metastasis (Aim 3). The application of these advances in nanotechnology to pre-clinical models and clinical subjects will lead to better understanding and treatment of cancer.