Metabolic Control Analysis

我们开发了一套使用CE和GC质谱分析代谢物的系统，适用于小组织样品的测量。在定义的途径中分析代谢物，并根据氧化还原和磷酸化状态的主要能量参数的变化查看数据。我们研究了饲喂酮酯饲料对脑中介代谢的影响。我们发现，喂养酮体增加了脑内ATP水解的能量，这可能对增加神经细胞离子梯度的程度有重要意义，这可能部分解释了酮症在某些耐药癫痫病例中减少癫痫发作的能力。另外，喂食酮类会增加脑丙二醇辅酶a，这可能使这种方法在治疗肥胖和相关疾病（如II型糖尿病）方面有用。

We have developed a system of analysis of metabolites using CE and GC mass spectroscopy suitable for measurement of small tissue samples. Metabolites in defined pathways are analyzed and the data viewed in terms of the variations in the major energetic parameters of redox and phosphorylation states. We have examined the effects of feeding ketone ester diets upon brain intermediary metabolism. We found that feeding ketone bodies increased the energy of ATP hydrolysis in brain, which may have significance in increasing the extent of ionic gradients in nerve cells, which is likely to account in part for the ability of ketosis to decrease epileptic episodes in some cases of drug resistant epilepsy. Additionally feeding ketones increased brain malonyl CoA, which may make this approach useful in the treatment of obesity and related diseases such as type II diabetes. A practical dietary regime has been developed which may be of clinical use in the treatment of very common diseases affecting the state of public health in the United States and elsewhere in the developed world.