Heart/Cell Function Core

核心C，心脏/细胞功能核心，将服务于本计划项目资助中的项目1-3，以实现 以下功能： 1.通过永久性或暂时性心肌梗死建立可靠、经济的小鼠心肌梗死模型 结扎冠状动脉左前降支这些模型将是测试 在所有项目中进行治疗，为大型哺乳动物（猪）的进一步试验提供概念验证 模型; 2.通过超声心动图（每周一次）和有创超声心动图研究MI损伤后体内心功能的变化， 心室内血液动力学测量（在每次研究结束时）。这些测量将提供 所有治疗有效性的可重复评估; 3.分离和表征心肌细胞功能，以探讨潜在的细胞机制。 每个项目中提出的治疗方式的有益效果。 4.集中每个项目的组织和细胞样本（小鼠和猪）。在这个核心中，我们将修复或冻结 组织或分离的细胞样品。这些样本将被收集并保存在 集中和维护良好的设施。将根据要求为每个项目分发样品。 5.为所有项目准备组织学样本。 6.准备用于分子分析的组织，并在需要时进行这些研究。 总之。核心C将为本项目中的所有项目提供必要的支持， 既经济又科学、可靠、高效。 相关性（参见说明）： 心肌梗死是一个严重的健康问题，很少有有效的治疗方法来减缓或逆转心肌梗死。 病理性的结构和功能重塑。该核心将产生可靠的小鼠模型 供项目研究人员研究的系统。该核心还将制备来自猪的细胞和组织（核心B） 为所有3个项目的调查人员提供了培训。

Core C, the Heart/Cell Function Core, is going to serve projects 1-3 in this Program Project Grant to fulfill the following functions: 1. To establish reliable and economical mouse myocardial infarction models by permanently or temporarily ligating the left anterior descending coronary artery. These models will be the flatform for testing the therapeutical treatments in all projects to offer proof-of-concepts for further tests in large mammal (porcine) models; 2. To study in vivo cardiac function changes after Ml injury by echocardiography (weekly) and invasive intraventricular hemodynamic measurements (at the end of each study). These measurements will offer reproducible assessments of the efficacy of all treatments; 3. To isolate and characterize cardiac myocyte function to explore the cellular mechanisms of the potential beneficial effects of the therapeutical modalities as proposed in each project. 4. To centralize tissue and cell samples (mouse and pig) for each project. In this core, we will fix or freeze tissue or isolated cell samples after the treatment is done. These samples will be collected and kept in a centralized and well-maintained facility. The samples will be distributed for each project upon request. 5. To prepare histological samples for all projects. 6. To prepare tissues for molecular analysis and perform these studies when needed. In summary. Core C will provide essential support for all projects in this Project Program Project in an economical while scientifically reliable and efficient way. RELEVANCE (See instructions): Myocardial infarction is a significant health problem with few effective therapies to slow or reverse the pathological structural and functional remodeling that it induces. This core will produce reliable mouse model systems for investigators in projects to study. This core will also prepare cells and tissues from pigs (Core B) for investigators in all 3 projects.