Candida-Bacteria Communication in Oral Biofilms

描述（由申请方提供）：在口腔表面发现多微生物群落作为生物膜，包埋在宿主唾液成分和微生物胞外产物的基质中。这些群落为口腔硬组织和软组织疾病以及全身性疾病提供了感染源。微生物之间的交流反应调节群落的结构、组成和致病潜力。口腔链球菌在这方面尤其重要，因为它们是口腔表面的主要定植者。它们的沉积为潜在致病性生物体如牙龈卟啉单胞菌和真菌白色念珠菌的定植提供了附着基质，这是人类中大多数酵母感染的原因。C.白念珠菌和口腔细菌的存在促进了念珠菌的口腔携带和持续存在。白色念珠菌，导致疾病状况，如假牙引起的口腔炎、口腔念珠菌病、牙周炎和根管感染。研究表明，戈登链球菌和C。白色念珠菌涉及互补的和协同的粘附素受体分子。链球菌细胞表面相关SspB粘附素是抗原I/II蛋白家族的成员，结合C.白念珠菌菌丝细胞表面糖蛋白Als 3 p，导致 表面分子与伴侣细胞类型的紧密结合。这些粘附过程驱动在形态学、生理学和病理学上独特的混合物种生物膜的发展和积累。本研究的目的是：明确C.白色念珠菌表面蛋白与S. gordonii;表征多微生物群落发育中发生的信号传导过程;确定C.白色念珠菌和牙龈卟啉单胞菌;研究体内共定植的影响。这些目标，同时涵盖了一系列的因素，将允许详细分析的分子通信系统之间发生的共同殖民的细菌和真菌。对义齿材料、口腔粘膜和龈下组织共定植机制的表征，将使我们更好地了解这些部位与多种微生物介导的口腔疾病发生和发展相关的因素。因此，可以确定抑制共粘附和群落发育的潜在靶点，这些靶点最终可能被证明是控制C.白色念珠菌

DESCRIPTION (provided by applicant): Polymicrobial communities are found on oral cavity surfaces as biofilms, embedded in a matrix of host salivary components and microbial extracellular products. These communities provide an infection source for diseases of the oral hard and soft tissues, and for systemic conditions. Communication reactions between the micro-organisms modulate the structure, composition and pathogenic potential of the communities. The oral streptococci are especially important in this respect because they are primary colonizers of oral cavity surfaces. Their deposition provides an attachment substrate for colonization by potentially pathogenic organisms such as Porphyromonas gingivalis and the fungus Candida albicans, which is the cause of most yeast infections in humans. Co-adhesion between C. albicans and oral bacteria is proposed to facilitate oral carriage and persistence of C. albicans, leading to disease conditions such as denture-induced stomatitis, oral candidiasis, periodontitis, and root canal infections. Studies have demonstrated that inter-microbial binding of Streptococcus gordonii and C. albicans involves complementary and co-operative adhesin-receptor molecules. Streptococcal cell surface-associated SspB adhesin, a member of the Antigen I/II protein family, binds C. albicans hyphal cell surface glycoprotein Als3p, resulting in close engagement of the surface molecules on the partner cell types. These adhesion processes drive the development and accumulation of mixed species biofilms that are morphologically, physiologically and pathogenically unique. The aims of this proposal are to: define the molecular mechanisms of C. albicans surface protein interactions with S. gordonii; characterize the signaling processes occurring in polymicrobial community development; determine the mechanisms of communication between C. albicans and P. gingivalis; investigate the impact of co-colonization in vivo. These aims, while covering a range of factors, will permit detailed analyses of molecular communication systems occurring between co-colonizing bacteria and fungi. Characterization of the mechanisms involved in co-colonization of denture materials, oral mucosa, and of sub-gingival tissues, will provide greater understanding of the factors relevant to polymicrobial-mediated oral disease initiation and progression at these sites. Potential targets for inhibition of co-adhesion and community development can thus be identified, and these may ultimately prove a means for the control of infection by C. albicans.