Neutralizing antibodies scientific research support component

中和抗体科学研究支助部分将为CHAVI提供基本的实验室和科学支持--在标准化和验证的分析中对针对第一级和第二级参考HLV-1毒株的中和活性进行高通量评估。此外，我们将绘制广泛中和血清和单抗的表位图，监测疫苗引发的NAB反应，以确定改进的免疫原、佐剂和载体，并将NAB描述为使用2b期试验中的突破性病毒进行保护的相关性。 具体目标 目的1.通过定义选定临床人群血清中和活性的大小和范围(M-B)，并绘制广泛中和血清的表位图，支持CHAVI-ID的研究。 目的2.筛选记忆性B细胞培养上清液以鉴定产生NAB的B细胞，以支持CHAVI-ID的研究。 目的3.通过鉴定新的单抗的中和活性和广度以及表位特异性来支持CHAVI-ID的研究。 目的4.通过监测动物和人类接种者中疫苗引起的NAB的大小和广度，以及评估疫苗血清与人类疫苗突破感染的病毒的中和作用，支持CHAVI-ID研究。

The Neutralizing Antibody Scientific Research Support Component will provide essential laboratory and scientific support for CHAVI- by performing high throughput assessments of neutralizing activity against tier 1 and tier 2 reference HlV-1 strains in standardized and validated assays. In addition, we will map the epitopes of broadly neutralizing sera and mAbs, monitor vaccine-elicited nAb responses to identify improved immunogens, adjuvants and vectors, and delineate nAbs as a correlate of protection using breakthrough viruses from phase 2b trials. Specific Aims Aim 1. To support CHAVI-ID research by defining the magnitude and breadth (M-B) of neutralizing activity in sera from select clinical groups, and by mapping the epitopes of broadly neutralizing sera. Aim 2. To support CHAVI-ID research by screening memory B cell culture supernatants to identify nAb-producing B cells. Aim 3. To support CHAVI-ID research by characterizing new mAbs for magnitude and breadth of neutralizing activity and epitope specificity. Aim 4. To support CHAVI-ID research by monitoring the magnitude and breadth of vaccine-elicited nAbs in animals, human vaccinees, and by assessing vaccine sera neutralization with viruses from vaccine breakthrough infections in humans.