Skin Cell Culture Core

作为DAB皮肤病研究中心(SDRC)的一部分，DAB SDRC皮肤细胞培养的使命 核心(SCCC)是提供有价值的资源、服务和研究技术培训，以促进 皮肤生物学的跨学科研究。在过去的4年里，我们的核心服务已经成为至关重要的 向由35名UAB SDRC成员和12名新调查人员组成的多元化社区提供资源，这些调查人员代表22名 依靠我们的专业知识促进和支持与皮肤生物学相关的研究的部门。 SCCC的教职员工和研究人员在生成和维护 纯化的表皮和真皮初级细胞亚群，主要来自小鼠和人类皮肤组织或 单层或器官培养的活组织检查样本。我们建议继续支持和推动 UAB的皮肤研究有以下具体目标： 1)提供从小鼠和人类组织到中心的表皮和真皮细胞类型的中央来源 以符合成本效益的方式为会员服务。 2)维持质量控制措施，以确保分发的标准化产品无污染 文化。 3)提供专门的细胞培养系统和皮肤细胞的转基因技术 人类或老鼠来源。 4)优化小鼠连续长期培养方法和永生化方案 来自野生型和突变小鼠品系的角质形成细胞通过以下方式适应增加的需求 发改委委员。 通过以鼓励新的探索和创新的成本提供各种类型的细胞，国家发改委 对提高质量和扩大皮肤研究的广度至关重要，由 在过去的4年里，有多名UAB调查人员。我们建议继续并改进这项重要的服务。

As part of the DAB Skin Diseases Research Center (SDRC), the mission of the DAB SDRC Skin Cell Culture Core (SCCC) is to provide valuable resources, services, and training in research techniques for promoting interdisciplinary research in cutaneous biology. Over the last 4 years, our core services have become a vital resource to a diverse community of 35 UAB SDRC members and 12 new investigators representing 22 departments who depend upon our expertise to facilitate and support studies relating to cutaneous biology. The SCCC faculty and research staff have established expertise in the generation and maintenance of purified epidermal and dermal primary cell subsets, derived primarily from murine and human skin tissues or biopsy samples, in monolayer or organotypic cultures. We propose to continue to support and promote cutaneous research at UAB with the following specific aims: 1) To provide a central source of epidermal and dermal cell types from murine and human tissues to Center members in a cost efficient manner. 2) To maintain quality control measures that ensure the distribution of standardized contamination-free cultures. 3) To provide expertise in generating specialized cell culture systems and transfecting skin cells from either human or mouse sources. 4) To optimize methods for continuous long-term culture and immortalization protocols of murine keratinocytes derived from wild type and mutant mouse strains to accommodate increased demand by SDRC members. By providing various cell types at a cost that encourages new exploration and innovation, the SDRC SCCC has been essential for enhancing the quality and expanding the breadth of cutaneous research performed by multiple UAB investigators during last 4 years. We propose to continue and improve upon this vital service.