Analytical Cytometry

OSUCCC分析细胞学共享资源(ACSR)是一项广泛的、得到制度支持的共享服务。该核心提供了快速而准确地分析生物颗粒的多种特征的唯一手段之一，同时还能够快速、准确且具有高纯度(&gt；98%)根据研究人员指定的参数分选出感兴趣的纯细胞群体。 此外，与磁珠技术相比，这项服务为OSUCCC成员提供了获得活的、无菌的和纯的细胞群体的能力，以便可以单独克隆它们，可以评估免疫功能，或者可以通过最少的操作来检查特定的生化特性。这个共享资源有五个主要目标：1)提供最先进的流式细胞术 这些研究包括：1)在收费的基础上进行分析和分类；2)为希望进行自己分析的研究人员提供个人培训，随后24小时使用流式细胞仪仪器；3)为新的和有经验的资源用户开发和提供教育和培训机会，以及向OSUCCC研究人员介绍新仪器、技术和试剂的论坛；4)获得并提供最先进的设备，以支持OSUCCC成员进行高质量的癌症研究；以及5)引入新的或商业化前的新兴技术，以支持OSUCCC成员进行高质量的癌症研究。ACSR主要设施位于中心位置，拥有8台流式细胞仪，其中4台能够分选。提供两台独立的流式细胞仪分析仪(24小时可用) 和辅助分析。此外，还提供商用和原型磁分离和分析设备。在过去四年中，这些文书中有五件是在机构支助下购买的，数额约为1,358,000美元。为了满足大量用户的需要，并保持足够的空间和通道，ACSR在詹姆斯癌症医院(JCH)和俄亥俄州立大学(OSU)设有两个卫星设施 兽医学院(CVM)CVM有三个流式细胞仪，其中一个配备了安全地对感染病毒的细胞进行分类。ACSR主任是杰弗里·查尔默斯博士，还有一名经理布莱恩·麦克尔文和另外两名员工。云服务器卫星由A·尼科尔·怀特管理，并有一名额外的技术人员。此外，在过去的一年里，玛丽·乔·伯克哈德博士被聘为 ACSR的重点是教育和外展。ACSR继续为研究人员和科学项目提供关键支持，包括14项积极使用ACSR服务的临床研究。在过去的一年里，近75%的ACSR使用来自63个CCSG同行评审、资助的所有六个项目的OSUCCC研究人员，他们花费了超过4300个小时的服务，但只有23.4%的支持来自CCSG。

The OSUCCC Analytical Cytometry Shared Resource (ACSR) is an extensive, institutionally-supported shared service. This core provides one of the only means of rapidly and accurately analyzing multiple characteristics of biological particles while also being able to rapidly, accurately, and with high purity (>98%) sort out pure populations of cells of interest based on parameters designated by the investigator. Furthermore, this service provides OSUCCC members with the ability to obtain viable, sterile and pure populations of cells so that they may be individually cloned, can be assessed for immunological function, or can be examined for specific biochemical properties with minimal manipulations, compared to magnetic bead technologies. This shared resource has five primary goals: 1) Provide state of the art flow cytometry analysis and sorting on a fee-for-service basis; 2) Provide individual training followed by 24-hour access to flow cytometry instrumentation for researchers who wish to conduct their own analysis; 3) Develop and provide educational and training opportunities for new and experienced resource users as well as forums to introduce new instrumentation, technologies and reagents to OSUCCC investigators; 4) Obtain and provide state-of-the-art equipment to support high quality cancer research for OSUCCC members; and 5) Introduce new, or pre-commercial, emerging technology to support high quality cancer research for OSUCCC members. The ACSR main facility is centrally located and has eight flow cytometry instruments, four of which are capable of sorting. Two flow cytometer analyzers are available for independent (24 hour access) and assisted analysis. In addition, commercial and prototype magnetic separation and analysis equipment is available. Five' of these instruments were purchased with institufional support of approximately $1,358,000 in the last four years. In order to meet the needs of heavy users and maintain adequate space and access, the ACSR has two satellite facilifies located in the James Cancer Hospital (JCH) and the OSU College of Veterinary Medicine (CVM). The CVM has three flow cytometers, one of which is equipped to safely sort virus infected cells. The ACSR Director is Jeffrey Chalmers, Ph.D. with a manager, Bryan McElwain, and two additional staff. The CVM satellite is managed by A. Nicole White and has an additional technician. In addition, this past year Mary Jo Burkhard, D.V.M., Ph.D. was recruited as a co-investigator in the ACSR focused on education and outreach. The ACSR continues to provide critical support to the investigators and scientific programs, including 14 clinical studies acfively using the services of the ACSR This past year, nearly 75% of the ACSR usage was from 63 CCSG peer-reviewed, funded OSUCCC investigators from all six programs who consumed over 4,300 hours of service, yet only 23.4% of the support came from the CCSG.